Immunohistochemistry (Frozen sections) abreview for Anti-NUMBL antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (retina, brain)
Specification
retina, brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA blocking Solution
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: invitrogen Alexa fluor conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Very strong staining in the retina and brain, seemingly at the membrane, at 4dpf. Sodium Citrate antigen retrieval was used.

Dr. Ryan Macdonald

Verified customer

Submitted Nov 17 2014

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