Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab5641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Western Blot: Use at an assay dependent dilution.
We would welcome any customer feedback about ab5641.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of embryonic neurogenesis. Also required postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. Negative regulator of NF-kappa-B signaling pathway. The inhibition of NF-kappa-B activation is mediated at least in part, by preventing MAP3K7IP2 to interact with polyubiquitin chains of TRAF6 and RIPK1 and by stimulating the 'Lys-48'-linked polyubiquitination and degradation of TRAF6 in cortical neurons.
Sequence similaritiesContains 1 PID domain.
DomainThe PTB domain is necessary for the inhibition of MAP3K7IP2-mediated activation of NF-kappa-B.
Cellular localizationCytoplasm. Symmetrically distributed throughout the cytoplasm in non dividing neuroblasts of the CNS.
- Information by UniProt
- CAG 3A antibody
- CAG3A antibody
- CTG 3a antibody
Lanes 1-2 : Anti-NUMBL antibody (ab5641) at 1 µg/ml
Lane 3 : anti-MYC Tag at 1/1000 dilution
Lanes 1 & 3 : HEK293 Lysate overexpressing Human Numbl with C-terminal MYC tag
Lane 2 : Mock transfected HEK293
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Primary incubations were for 1 hour
This product has been referenced in:
- Ren Z et al. Fibroblast growth factor-21 alleviates hypoxia/reoxygenation injury in H9c2 cardiomyocytes by promoting autophagic flux. Int J Mol Med 43:1321-1330 (2019). Read more (PubMed: 30664197) »
- Tokumitsu H et al. Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases. J Biol Chem 280:35108-18 (2005). Read more (PubMed: 16105844) »