Anti-Nup107 antibody (ab73290)
Key features and details
- Rabbit polyclonal to Nup107
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nup107 antibody
See all Nup107 primary antibodies -
Description
Rabbit polyclonal to Nup107 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC HumanICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human Nup107 (internal sequence). The immunogen is located within amino acids 370 - 420 (20 amino acid synthetic peptide) of NUP107.
Database link: P57740 -
Positive control
- A549 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC |
Human
|
ICC/IF |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).
|
|
ICC |
Use a concentration of 10 µg/ml.
|
Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa). |
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. -
Tissue specificity
Ubiquitously expressed in fetal and adult tissues. -
Involvement in disease
Nephrotic syndrome 11 -
Sequence similarities
Belongs to the nucleoporin Nup84/Nup107 family. -
Cellular localization
Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755). - Information by UniProt
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Database links
- Entrez Gene: 57122 Human
- Omim: 607617 Human
- SwissProt: P57740 Human
- Unigene: 524574 Human
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Alternative names
- 107 kDa nucleoporin antibody
- NU107_HUMAN antibody
- Nuclear pore complex protein Nup107 antibody
see all
Images
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Immunofluorescence of NUP107 in A549 cells using ab73290 at 10 ug/ml.
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Lane 1 : Anti-Nup107 antibody (ab73290) at 1 µg/ml
Lane 2 : Anti-Nup107 antibody (ab73290) at 2 µg/ml
All lanes : A549 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 106 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 194 kDa. We are unsure as to the identity of these extra bands. -
ab73290 at 10µg/ml staining Nup107 in A549 cells by Immunocytochemistry.
Protocols
Datasheets and documents
References (4)
ab73290 has been referenced in 4 publications.
- Ben-Yishay R et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530
- Gasset-Rosa F et al. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death. Neuron 102:339-357.e7 (2019). PubMed: 30853299
- Guan Y et al. Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity. J Cell Mol Med N/A:N/A (2018). PubMed: 30506890
- Kane M et al. Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Elife 7:N/A (2018). PubMed: 30084827