Anti-Nup107 antibody (ab73290)

Since you are doing mouse on mouse staining, I would recommend either using Fab fragments for blocking, or directly conjugating your primary antibody to a fluorescent secondary. If you want to use the Fab fragment method, I have attached the Abcam protocol for this and I have placed a few possible secondary antibodies that would work below: Secondary’s: ab96879 -Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed ab96881 - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), pre-adsorbed The alternative method is to directly conjugate the antibody to a fluorescent molecule. You can do this really easily using one of our EasyLink conjugation kits, the link below will give you more information on these kits: https://www.abcam.com/index.htmlpageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink Please let me know if there is anything else i can help you with or if I can help you select further IF reagents.

I am unable to provide tracking information as this antibody has not been shipped yet. Also, as you purchased the antibody from Cedarlane we have to ship the antibody to them first and they will then ship it to you. As I mentioned in the previous email, it should arrive early next week. If there is anything else I can help you with, please let me know.

Thank you for your reply. I have processed you request to receive ab24609 as a replacement for ab73290, which did not work as guaranteed in ICC/IF. As this is being shipped to Canada, I am hoping that it will arrive early next week but due to Customs we cannot guarantee a delivery date. If there is anything else I can help you with, please let me know.

Thank you for your reply. I found one order from 24th March 2011 that was shipped to the address you gave. Would that date be correct, as in your original email, you said you have ordered these antibodies in the last few months and I just want to make sure, I am not missing any other orders. If I can get the replacement antibody order processed today then you should have it by Monday/Tuesday next week, with customs however we cannot guarantee a time, as they may hold it up. I look forward to your reply.

Thank you for your reply. I would be happy to send you ab24609 as a replacement antibody, but if I remember correctly, you had emailed previously about concerns over this antibodies ability to recognize Nup107. As I said I more than happy to send you the antibody if that’s what you want, I just wanted to double check. All I need to process the replacement is the ordering details of the last order placed. Do you order through Cedarlane? I look forward to your reply. .

Thank you for providing the protocol information. I was just wandering if you use/or have tried a permeabilization step? I ask as since it is a nuclear protein, it is generally recommended to use a 0.25% Triton step. If you have already tried using the Triton step, then based off the information you have provided then it seems as though you have tried many different protocol variations, therefore I would be happy to send you one of other Nup107 antibodies in our catalogue. I look forward to your reply.