Anti-Nup107 antibody (ab73290)

Submit a review Q&A (6)References (1)

Overview

  • Product name
    Anti-Nup107 antibody
    See all Nup107 primary antibodies
  • Description
    Rabbit polyclonal to Nup107
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 20 amino acid peptide from near the center of human Nup107

  • Positive control
    • A549 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).
ICC Use a concentration of 10 µg/ml.

Target

  • Function
    Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
  • Tissue specificity
    Ubiquitously expressed in fetal and adult tissues.
  • Involvement in disease
    Nephrotic syndrome 11
  • Sequence similarities
    Belongs to the nucleoporin Nup84/Nup107 family.
  • Cellular localization
    Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 107 kDa nucleoporin antibody
    • NU107_HUMAN antibody
    • Nuclear pore complex protein Nup107 antibody
    • Nucleoporin 107kDa antibody
    • Nucleoporin Nup107 antibody
    • NUP107 antibody
    • NUP84 antibody
    • NUP84, yeast, homolog of antibody
    • OTTHUMP00000240604 antibody
    • OTTHUMP00000240606 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nup107 antibody (ab73290)
    Immunocytochemistry/ Immunofluorescence - Anti-Nup107 antibody (ab73290)
    Immunofluorescence of NUP107 in A549 cells using ab73290 at 10 ug/ml.

  • Western blot - Anti-Nup107 antibody (ab73290)
    Western blot - Anti-Nup107 antibody (ab73290)
    Lane 1 : Anti-Nup107 antibody (ab73290) at 1 µg/ml
    Lane 2 : Anti-Nup107 antibody (ab73290) at 2 µg/ml

    All lanes : A549 cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 106 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 194 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry - Anti-Nup107 antibody (ab73290)
    Immunocytochemistry - Anti-Nup107 antibody (ab73290)

    ab73290 at 10µg/ml staining Nup107 in A549 cells by Immunocytochemistry.

References

This product has been referenced in:
  • Guan Y  et al. Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity. J Cell Mol Med N/A:N/A (2018). Read more (PubMed: 30506890) »
See 1 Publication for this product

Publishing research using ab73290? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

Can you suggest a secondary antibody for this product for IF? I will looking for expression in mouse itself. I have seen a paper that used this product on mouse neuronal cells but was unable to determine their secondary. Since this is mouse on mouse, do we need one of the Fab fragments?

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Answer

Since you are doing mouse on mouse staining, I would recommend either using Fab fragments for blocking, or directly conjugating your primary antibody to a fluorescent secondary. If you want to use the Fab fragment method, I have attached the Abcam protocol for this and I have placed a few possible secondary antibodies that would work below: Secondary’s: ab96879 -Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed ab96881 - Goat polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 594), pre-adsorbed The alternative method is to directly conjugate the antibody to a fluorescent molecule. You can do this really easily using one of our EasyLink conjugation kits, the link below will give you more information on these kits: https://www.abcam.com/index.htmlpageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink Please let me know if there is anything else i can help you with or if I can help you select further IF reagents.

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Question

Hello John, Can you provide me the tracking information. Many thanks

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Answer

I am unable to provide tracking information as this antibody has not been shipped yet. Also, as you purchased the antibody from Cedarlane we have to ship the antibody to them first and they will then ship it to you. As I mentioned in the previous email, it should arrive early next week. If there is anything else I can help you with, please let me know.

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Question

I checked with our administration office, our orders go via Cedar Lane - Purchase order number: ****, Invoice: **** Let me know on the shipping details. Many thanks,*

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Answer

Thank you for your reply. I have processed you request to receive ab24609 as a replacement for ab73290, which did not work as guaranteed in ICC/IF. As this is being shipped to Canada, I am hoping that it will arrive early next week but due to Customs we cannot guarantee a delivery date. If there is anything else I can help you with, please let me know.

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Question

do not have the order details on hand but here are some details, can you look it up?   My main goal is to localize the nuclear membrane, so the alternate antibody I mentioned will be fine. It does not recognize Nup107 but does characterize the nuclear membrane which is fine.    If the replacement request goes in today or tomorrow, when would it be shipped?  

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Answer

Thank you for your reply. I found one order from 24th March 2011 that was shipped to the address you gave. Would that date be correct, as in your original email, you said you have ordered these antibodies in the last few months and I just want to make sure, I am not missing any other orders. If I can get the replacement antibody order processed today then you should have it by Monday/Tuesday next week, with customs however we cannot guarantee a time, as they may hold it up. I look forward to your reply.

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Question

Hi John, Yes permeabilization step is a must and yes it was done with 0.25% Triton Since Nup107 (ab72390) two batches have not worked best for me. I was wondering if you substitute and send me the mAb414? https://www.abcam.com/Nuclear-Pore-Complex-Proteins-antibody-Mab414-ChIP-Grade-ab24609.html What would you need in order to process the shipment. I wanted to get the antibody before we close for the break in order for me to optimize and get it running before the new year!! Let me know.. Many thanks

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Answer

Thank you for your reply. I would be happy to send you ab24609 as a replacement antibody, but if I remember correctly, you had emailed previously about concerns over this antibodies ability to recognize Nup107. As I said I more than happy to send you the antibody if that’s what you want, I just wanted to double check. All I need to process the replacement is the ordering details of the last order placed. Do you order through Cedarlane? I look forward to your reply. .

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Question

I do not have the antibody lot details for the previous batch (as the vial was thrown) but it is the same catalogue number I have tried the following variables 1)3x Wash PBS 2)Block in 5% Milk/PBS, 10% Milk/PBS, 1%BSA, 3%BSA 3)Incubation with primary antibody - abcam Antibody concentration - 1:100 to 1:300 in 1xPBS, 5% Milk/PBS, 10% Milk/PBS, 1%BSA/PBS, 3% BSA/PBS 1 hour to 2 hours RT to O/N 4degrees 4) Wash PBS 3x 5) Secondary antibody (works perfect for other signals (CTCF, etc) 1:400 to 1:1000 - 1 hour 6) wash pbs 10min x 3 7) mount vectashield

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Answer

Thank you for providing the protocol information. I was just wandering if you use/or have tried a permeabilization step? I ask as since it is a nuclear protein, it is generally recommended to use a 0.25% Triton step. If you have already tried using the Triton step, then based off the information you have provided then it seems as though you have tried many different protocol variations, therefore I would be happy to send you one of other Nup107 antibodies in our catalogue. I look forward to your reply.

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