Key features and details
- Rabbit polyclonal to Nup107
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nup107 antibody
See all Nup107 primary antibodies
DescriptionRabbit polyclonal to Nup107
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residues 875 and 925 of Human Nup107 (NP_065134.1)
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85916 was affinity purified using an epitope specific to Nup107 immobilized on solid support.
Our Abpromise guarantee covers the use of ab85916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionPlays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
Tissue specificityUbiquitously expressed in fetal and adult tissues.
Involvement in diseaseNephrotic syndrome 11
Sequence similaritiesBelongs to the nucleoporin Nup84/Nup107 family.
Cellular localizationNucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
- Information by UniProt
- 107 kDa nucleoporin antibody
- NU107_HUMAN antibody
- Nuclear pore complex protein Nup107 antibody
All lanes : Anti-Nup107 antibody (ab85916) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutes
Detection of Nup107 by Western Blot of Immunprecipitate.
ab85916 at 1µg/ml staining Nup107 in HeLa whole cell lysate immunoprecipitated using ab85916 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab85916 has been referenced in 2 publications.
- Yokoyama H et al. The nucleoporin MEL-28 promotes RanGTP-dependent ?-tubulin recruitment and microtubule nucleation in mitotic spindle formation. Nat Commun 5:3270 (2014). PubMed: 24509916
- Light WH et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). PubMed: 23555195