Recombinant Anti-Nup107 antibody [EPR12242] (ab178399)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12242] to Nup107
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Nup107 antibody [EPR12242]
See all Nup107 primary antibodies -
Description
Rabbit monoclonal [EPR12242] to Nup107 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Nup107 aa 750-850 (Cysteine residue). The exact sequence is proprietary.
Database link: P57740 -
Positive control
- 293T, HeLa and BxPC-3 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12242 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 106 kDa.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 106 kDa. |
Target
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Function
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. -
Tissue specificity
Ubiquitously expressed in fetal and adult tissues. -
Involvement in disease
Nephrotic syndrome 11 -
Sequence similarities
Belongs to the nucleoporin Nup84/Nup107 family. -
Cellular localization
Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755). - Information by UniProt
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Database links
- Entrez Gene: 57122 Human
- Entrez Gene: 116555 Rat
- Omim: 607617 Human
- SwissProt: P57740 Human
- SwissProt: P52590 Rat
- Unigene: 524574 Human
- Unigene: 11398 Rat
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Alternative names
- 107 kDa nucleoporin antibody
- NU107_HUMAN antibody
- Nuclear pore complex protein Nup107 antibody
see all
Images
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All lanes : Anti-Nup107 antibody [EPR12242] (ab178399) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 106 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab178399 has been referenced in 2 publications.
- Platani M et al. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci 131:N/A (2018). PubMed: 29618633
- Otsuka S et al. Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope. Elife 5:N/A (2016). PubMed: 27630123