Overview

  • Product name

    Anti-Nup107 antibody [EPR12242]
    See all Nup107 primary antibodies
  • Description

    Rabbit monoclonal [EPR12242] to Nup107
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Nup107 aa 750-850 (Cysteine residue). The exact sequence is proprietary.
    Database link: P57740

  • Positive control

    • 293T, HeLa and BxPC-3 cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 106 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC.
    • Tissue specificity

      Ubiquitously expressed in fetal and adult tissues.
    • Involvement in disease

      Nephrotic syndrome 11
    • Sequence similarities

      Belongs to the nucleoporin Nup84/Nup107 family.
    • Cellular localization

      Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755).
    • Information by UniProt
    • Database links

    • Alternative names

      • 107 kDa nucleoporin antibody
      • NU107_HUMAN antibody
      • Nuclear pore complex protein Nup107 antibody
      • Nucleoporin 107kDa antibody
      • Nucleoporin Nup107 antibody
      • NUP107 antibody
      • NUP84 antibody
      • NUP84, yeast, homolog of antibody
      • OTTHUMP00000240604 antibody
      • OTTHUMP00000240606 antibody
      see all

    Images

    • All lanes : Anti-Nup107 antibody [EPR12242] (ab178399) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 106 kDa

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted May 24 2018

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