Recombinant Anti-NUP133 antibody [EPR10808(B)] (ab155990)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10808(B)] to NUP133
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
Related conjugates and formulations
Overview
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Product name
Anti-NUP133 antibody [EPR10808(B)]
See all NUP133 primary antibodies -
Description
Rabbit monoclonal [EPR10808(B)] to NUP133 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Ramos and SH-SY5Y cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10808(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 129 kDa.
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ICC/IF | (1) |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 129 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Involved in poly(A)+ RNA transport. -
Sequence similarities
Belongs to the nucleoporin Nup133 family. -
Cellular localization
Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores. - Information by UniProt
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Database links
- Entrez Gene: 55746 Human
- Omim: 607613 Human
- SwissProt: Q8WUM0 Human
- Unigene: 12457 Human
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Alternative names
- 133 kDa nucleoporin antibody
- FLJ10814 antibody
- hNUP 133 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling NUP133 with purified ab155990 at 1:50 (6.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (purified) + COS-1 (African green monkey kidney fibroblast-like) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 129 kDa
Observed band size: 129 kDa -
Lanes 1 & 3 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purified)
Lane 2 : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/3000 dilution (purifed)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lane 3 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 129 kDa
Observed band size: 129 kDa -
All lanes : Anti-NUP133 antibody [EPR10808(B)] (ab155990) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 129 kDaThis image was produced using unpurified antibody.
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Unpurified ab155990 staining NUP133 in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. This image was produced using unpurified antibody.
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Immunofluorescent analysis of HeLa cells labeling NUP133 with unpurified ab155990 at 1/50 dilution. This image was produced using unpurified antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab155990 has been referenced in 7 publications.
- Mammel AE et al. Chromosome length and gene density contribute to micronuclear membrane stability. Life Sci Alliance 5:N/A (2022). PubMed: 34789512
- Chachoua I et al. Canonical WNT signaling-dependent gating of MYC requires a noncanonical CTCF function at a distal binding site. Nat Commun 13:204 (2022). PubMed: 35017527
- Chou YY et al. Inherited nuclear pore substructures template post-mitotic pore assembly. Dev Cell 56:1786-1803.e9 (2021). PubMed: 34129835
- Bilir S et al. Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis. Genes Cells 24:338-353 (2019). PubMed: 30821042
- Platani M et al. Seh1 targets GATOR2 and Nup153 to mitotic chromosomes. J Cell Sci 131:N/A (2018). PubMed: 29618633
- Aristov A et al. ZOLA-3D allows flexible 3D localization microscopy over an adjustable axial range. Nat Commun 9:2409 (2018). PubMed: 29921892
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450