Immunocytochemistry/ Immunofluorescence abreview for Anti-NUP133 antibody [EPR10808(B)]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
3% BSA and 0.5% TX100 as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Monkey Cell (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
45 minute(s) · Temperature: 25°C · Diluent: 3% BSA and 0.5% TX100
Dilution
1/1500

Secondary antibody

Concentration
2 µg/ml
Name
Non-Abcam antibody was used: Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 647

Additional data

Additional Notes
3% Paraformaldehyde only fixation. PFA/GA fixation did not yield good images.

Dr. Aaron Halpen

Verified customer

Submitted Sep 30 2014

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