Recombinant
RabMAb

Recombinant Anti-NUP133 antibody [EPR10808(B)] (Alexa Fluor® 647) (ab225215)

Overview

  • Product name
    Anti-NUP133 antibody [EPR10808(B)] (Alexa Fluor® 647)
    See all NUP133 primary antibodies
  • Description
    Rabbit monoclonal [EPR10808(B)] to NUP133 (Alexa Fluor® 647)
  • Host species
    Rabbit
  • Conjugation
    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NUP133.

  • Positive control
    • ICC/IF: Ramos cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.

This product gave a positive signal in Ramos cells fixed with 4% formaldehyde (10 min)

Target

  • Function
    Involved in poly(A)+ RNA transport.
  • Sequence similarities
    Belongs to the nucleoporin Nup133 family.
  • Cellular localization
    Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Located on both the cytoplasmic and nuclear sides of the nuclear pore. During mitosis, localizes to the kinetochores.
  • Information by UniProt
  • Database links
  • Alternative names
    • 133 kDa nucleoporin antibody
    • FLJ10814 antibody
    • hNUP 133 antibody
    • hNUP133 antibody
    • MGC21133 antibody
    • NU133_HUMAN antibody
    • Nuclear pore complex protein Nup133 antibody
    • Nucleoporin 133kDa antibody
    • Nucleoporin NUP133 antibody
    • NUP 133 antibody
    • NUP133 antibody
    • OTTHUMP00000061095 antibody
    see all

Images

  • ab225215 staining NUP133 in Ramos cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225215 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225215 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

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