Key features and details
- Rabbit polyclonal to Nup153
- Suitable for: IP, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nup153 antibody
See all Nup153 primary antibodies
DescriptionRabbit polyclonal to Nup153
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Nup153 aa 50-100. The exact sequence is proprietary. NP_005115.2
Database link: P49790
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. ICC/IF: Asynchronous HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245668 was affinity purified using an epitope specific to Nup153 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 154 kDa.|
|ICC/IF||1/100 - 1/500.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
FunctionPossible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Sequence similaritiesContains 4 RanBP2-type zinc fingers.
DomainContains F-X-F-G repeats.
Cellular localizationNucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
- Information by UniProt
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
Formaldehyde-fixed asynchronous HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Nup153 using ab245668 at a 1/100 dilution in ICC/IF.
Detection: Red-fluorescent goat anti-rabbit IgG, highly cross-absorbed.
All lanes : Anti-Nup153 antibody (ab245668) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 154 kDa
Exposure time: 3 minutes
Nup153 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245668 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245668 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245668 has not yet been referenced specifically in any publications.