Key features and details
- Rabbit polyclonal to Nup153
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nup153 antibody
See all Nup153 primary antibodies
DescriptionRabbit polyclonal to Nup153
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Nup153 aa 1425-1475. The exact sequence is proprietary. NP_005115.2
Database link: P49790
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245669 was affinity purified using an epitope specific to Nup153 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 154 kDa.|
FunctionPossible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Sequence similaritiesContains 4 RanBP2-type zinc fingers.
DomainContains F-X-F-G repeats.
Cellular localizationNucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
- Information by UniProt
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
All lanes : Anti-Nup153 antibody (ab245669) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 154 kDa
Exposure time: 3 minutes
Nup153 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245669 for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245669 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245669 has not yet been referenced specifically in any publications.