Anti-Nup153 antibody (ab84872)
Key features and details
- Rabbit polyclonal to Nup153
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nup153 antibody
See all Nup153 primary antibodies -
Description
Rabbit polyclonal to Nup153 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residue 50 and 100 of human Nup153 (NP_005115.2).
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General notes
Concentration is optimized for IHC and not determined
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to NUP153 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
1/100 - 1/500.
Formaldehyde fixed cells. Permeabilization with TritonX-100 is recommended. Acetone fixation is not recommended. |
WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa. PubMed: 21858095
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Notes |
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ICC/IF
1/100 - 1/500. Formaldehyde fixed cells. Permeabilization with TritonX-100 is recommended. Acetone fixation is not recommended. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa. PubMed: 21858095 |
Target
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Function
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
Sequence similarities
Contains 4 RanBP2-type zinc fingers. -
Domain
Contains F-X-F-G repeats. -
Cellular localization
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
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Database links
- Entrez Gene: 9972 Human
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
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Alternative names
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
Images
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Staining of human NUP153 in NBF fixed, asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
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Staining of human NUP153 in NBF fixed asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red-fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab84872 has been referenced in 19 publications.
- Golchoubian B et al. Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation. J Cell Biol 221:N/A (2022). PubMed: 34874453
- Damstra HGJ et al. Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx). Elife 11:N/A (2022). PubMed: 35179128
- Lv J et al. Gasdermin E mediates resistance of pancreatic adenocarcinoma to enzymatic digestion through a YBX1-mucin pathway. Nat Cell Biol 24:364-372 (2022). PubMed: 35292781
- Gleixner AM et al. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun 13:3380 (2022). PubMed: 35697676
- Singh SP et al. Viral protein X unlocks the nuclear pore complex through a human Nup153-dependent pathway to promote nuclear translocation of the lentiviral genome. Mol Biol Cell 31:304-317 (2020). PubMed: 31913756