Product nameAnti-Nup153 antibody
See all Nup153 primary antibodies
DescriptionRabbit polyclonal to Nup153
Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 50 and 100 of human Nup153 (NP_005115.2).
- Human HeLa cells
Concentration is optimized for IHC and not determined
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified using an epitope specific to NUP153 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Our Abpromise guarantee covers the use of ab84872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500. (formaldehyde fixed cells). Permeabilization with Triton X 100 is recommended. Acetone fixation is not recommended.|
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. PubMed: 21858095|
FunctionPossible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Sequence similaritiesContains 4 RanBP2-type zinc fingers.
DomainContains F-X-F-G repeats.
Cellular localizationNucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
- Information by UniProt
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
All lanes : Anti-Nup153 antibody (ab84872) at 1/1000 dilution
Lane 1 : U2OS cell lysate - treated with DMSO
Lane 2 : U2OS cell lysate - treated with H2O2
Lysates/proteins at 20 µg per lane.
All lanes : IRDye® 800CW-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 153 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Staining of human NUP153 in NBF fixed, asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
Staining of human NUP153 in NBF fixed asynchronous HeLa cells by Immunocytochemistry, using ab84872 at a dilution of 1/250. Detection: Red-fluorescent goat anti-rabbit IgG used at a dilution of 1/100.
This product has been referenced in:
- Dharan A et al. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog 12:e1005700 (2016). Read more (PubMed: 27327622) »
- Au S et al. A new mechanism for nuclear import by actin-based propulsion used by a baculovirus nucleocapsid. J Cell Sci 129:2905-11 (2016). Read more (PubMed: 27284005) »