Overview

  • Product name
    Anti-Nup153 antibody [EPR7606(2)(B)]
    See all Nup153 primary antibodies
  • Description
    Rabbit monoclonal [EPR7606(2)(B)] to Nup153
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P49790

  • Positive control
    • HeLa, K562 and HepG2 cell lysates. K562 and HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
    • Sequence similarities
      Contains 4 RanBP2-type zinc fingers.
    • Domain
      Contains F-X-F-G repeats.
    • Cellular localization
      Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
    • Information by UniProt
    • Database links
    • Alternative names
      • 153 kDa nucleoporin antibody
      • HNUP153 antibody
      • N153 antibody
      • NU153_HUMAN antibody
      • Nuclear pore complex protein hnup153 antibody
      • Nuclear pore complex protein Nup153 antibody
      • Nucleoporin 153kDa antibody
      • Nucleoporin Nup153 antibody
      • Nup 153 antibody
      • Nup153 antibody
      see all

    Images

    • All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 154 kDa

    • Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.

    • Flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab171074 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab171074.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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