Product nameAnti-Nup153 antibody [EPR7606(2)(B)]
See all Nup153 primary antibodies
DescriptionRabbit monoclonal [EPR7606(2)(B)] to Nup153
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P49790
- HeLa, K562 and HepG2 whole cell lysate (ab7900). K562 and HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 154 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPossible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Sequence similaritiesContains 4 RanBP2-type zinc fingers.
DomainContains F-X-F-G repeats.
Cellular localizationNucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
- Information by UniProt
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 154 kDa
Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.
Flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
ab171074 has not yet been referenced specifically in any publications.