Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Nup153 antibody [EPR7606(2)(B)]
See all Nup153 primary antibodies -
DescriptionRabbit monoclonal [EPR7606(2)(B)] to Nup153
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P49790 -
Positive control
- HeLa, K562 and HepG2 cell lysates. K562 and HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR7606(2)(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab171074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 154 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionPossible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
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Sequence similaritiesContains 4 RanBP2-type zinc fingers.
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DomainContains F-X-F-G repeats.
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Cellular localizationNucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
- Information by UniProt
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Database links
- Entrez Gene: 9972 Human
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
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Alternative names
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
Images
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![Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-171851-WBab171074.jpg)
Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 154 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-171852-ICCab171074.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.
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![Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-171853-flowab171074.jpg)
Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)Flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
References
ab171074 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"