Anti-Nup153 antibody [EPR7606(2)(B)] Recombinant (ab171074)

Product name

Anti-Nup153 antibody [EPR7606(2)(B)]
See all Nup153 primary antibodies
Description

Rabbit monoclonal [EPR7606(2)(B)] to Nup153
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat
Immunogen

Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P49790
Positive control
- HeLa, K562 and HepG2 cell lysates. K562 and HeLa cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7606(2)(B)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab171074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
ICC/IF		1/100 - 1/250.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application

Abreviews

Notes

WB

1/1000 - 1/10000. Predicted molecular weight: 154 kDa.

ICC/IF

1/100 - 1/250.

Flow Cyt

1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IHC-P or IP.

Function

Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Sequence similarities

Contains 4 RanBP2-type zinc fingers.
Domain

Contains F-X-F-G repeats.
Cellular localization

Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
Target information above from: UniProt accession P49790 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9972 Human
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
Alternative names
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
- NU153_HUMAN antibody
- Nuclear pore complex protein hnup153 antibody
- Nuclear pore complex protein Nup153 antibody
- Nucleoporin 153kDa antibody
- Nucleoporin Nup153 antibody
- Nup 153 antibody
- Nup153 antibody
see all

Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 154 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.
Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

Flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

ab171074 has not yet been referenced specifically in any publications.

Publishing research using ab171074? Please let us know so that we can cite the reference in this datasheet.

Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)