Recombinant Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
![Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-473336-anti-nup153-antibody-epr76062b-immunocytochemistry-immunofluorescence-hela-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7606(2)(B)] to Nup153
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Nup153 antibody [EPR7606(2)(B)]
See all Nup153 primary antibodies -
Description
Rabbit monoclonal [EPR7606(2)(B)] to Nup153 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P49790 -
Positive control
- WB: HeLa, K562 and HepG2 whole cell lysate (ab7900). Flow Cyt (intra): K562 ICC: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7606(2)(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
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| ICC/IF |
1/100 - 1/250.
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 154 kDa. |
|
ICC/IF
1/100 - 1/250. |
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
Sequence similarities
Contains 4 RanBP2-type zinc fingers. -
Domain
Contains F-X-F-G repeats. -
Cellular localization
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
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Database links
- Entrez Gene: 9972 Human
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
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Alternative names
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
ab171074 staining Nup153 in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab171074 at 0.4 µg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 µg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 µg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
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![Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-171851-anti-nup153-antibody-epr76062b-western-blot.jpg)
Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 154 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-171852-anti-nup153-antibody-epr76062b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.
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![Flow Cytometry (Intracellular) - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-171853-anti-nup153-antibody-epr76062b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)Intracellular flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)](https://www.abcam.com/ps/products/171/ab171074/Images/ab171074-4-benefits-of-recombinant-antibodies.png)
Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab171074 has been referenced in 1 publication.
- Wong A et al. Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment. Cell Death Discov 4:109 (2018). PubMed: 30510774
