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Tags & Cell Markers Subcellular Markers Nucleus Nuclear Pore
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RecombinantRabMAb

Recombinant Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

  • Datasheet
  • SDS
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Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
  • Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
  • Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7606(2)(B)] to Nup153
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-Nup153 antibody [EPR7606(2)(B)]
    See all Nup153 primary antibodies
  • Description

    Rabbit monoclonal [EPR7606(2)(B)] to Nup153
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P49790

  • Positive control

    • HeLa, K562 and HepG2 whole cell lysate (ab7900). K562 and HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7606(2)(B)
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

Associated products

  • Alternative Versions

    • Anti-Nup153 antibody [EPR7606(2)(B)] - BSA and Azide free (ab249630)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
    • Sequence similarities

      Contains 4 RanBP2-type zinc fingers.
    • Domain

      Contains F-X-F-G repeats.
    • Cellular localization

      Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
    • Target information above from: UniProt accession P49790 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9972 Human
      • Omim: 603948 Human
      • SwissProt: P49790 Human
      • Unigene: 601591 Human
      • Unigene: 718703 Human
      • Alternative names

        • 153 kDa nucleoporin antibody
        • HNUP153 antibody
        • N153 antibody
        • NU153_HUMAN antibody
        • Nuclear pore complex protein hnup153 antibody
        • Nuclear pore complex protein Nup153 antibody
        • Nucleoporin 153kDa antibody
        • Nucleoporin Nup153 antibody
        • Nup 153 antibody
        • Nup153 antibody
        see all

      Images

      • Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 154 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

        Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.

      • Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

        Flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

      • Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab171074? Please let us know so that we can cite the reference in this datasheet.

      ab171074 has been referenced in 1 publication.

      • Wong A  et al. Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment. Cell Death Discov 4:109 (2018). PubMed: 30510774

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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