RecombinantRabMAb

Recombinant Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

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Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
  • Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
  • Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7606(2)(B)] to Nup153
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Nup153 antibody [EPR7606(2)(B)]
    See all Nup153 primary antibodies

  • Description

    Rabbit monoclonal [EPR7606(2)(B)] to Nup153

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human Nup153 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P49790

  • Positive control

    • HeLa, K562 and HepG2 whole cell lysate (ab7900). K562 and HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 154 kDa.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.

    • Sequence similarities

      Contains 4 RanBP2-type zinc fingers.

    • Domain

      Contains F-X-F-G repeats.

    • Cellular localization

      Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
    • Information by UniProt

    • Database links

      • Alternative names

        • 153 kDa nucleoporin antibody
        • HNUP153 antibody
        • N153 antibody
        • NU153_HUMAN antibody
        • Nuclear pore complex protein hnup153 antibody
        • Nuclear pore complex protein Nup153 antibody
        • Nucleoporin 153kDa antibody
        • Nucleoporin Nup153 antibody
        • Nup 153 antibody
        • Nup153 antibody
        see all

      Images

      • Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Western blot - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        All lanes : Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 154 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

        Immunofluorescent analysis of HeLa cells labeling Nup153 with ab171074 at 1/100 dilution.

      • Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Flow Cytometry - Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

        Flow cytometric analysis of permeabilized K562 cells labeling Nup153 with ab171074 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

      • Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)
        Anti-Nup153 antibody [EPR7606(2)(B)] (ab171074)

      References (1)

      Publishing research using ab171074? Please let us know so that we can cite the reference in this datasheet.

      ab171074 has been referenced in 1 publication.

      • Wong A  et al. Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment. Cell Death Discov 4:109 (2018). PubMed: 30510774

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