Overview

  • Product name
    Anti-Nup153 antibody [nup7A8]
    See all Nup153 primary antibodies
  • Description
    Mouse monoclonal [nup7A8] to Nup153
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis, Newt
  • Immunogen

    Nuclear matrix protein fraction prepared from Rat liver nuclei.

Properties

Applications

Our Abpromise guarantee covers the use of ab93310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/5 - 1/10.
WB 1/30 - 1/50. Predicted molecular weight: 154 kDa.
IP 1/5 - 1/10.

Target

References

This product has been referenced in:
  • Re A  et al. Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer. Oncotarget 9:27985-27997 (2018). Read more (PubMed: 29963256) »
  • Harhouri K  et al. MG132-induced progerin clearance is mediated by autophagy activation and splicing regulation. EMBO Mol Med 9:1294-1313 (2017). ICC/IF . Read more (PubMed: 28674081) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your patience.

You have spoken last week to my colleague in regards to two bands observed with the ab93310. He has contacted the laboratory to ask for possible explanations. As he is out of the office this week, I am transmitting you now the reply.

Indeed, the expert on Nup153 answered that "the apparent molecular weight observed depends largely on the gel system applied. A band at 190 kDa may be observed in Thomas-Kornberg type gels. A difference of 30 kD between individual references was observed (e.g. in Laemmli-type gels) and accepted. Additionally, the observation of 2 close (154/157 kDa) immunoreactive polypeptide bands is not new and reflects most probably the quality of the electrophoretic separation conditions and also depends on the species investigated. There is also the possibility of post-translational modification of the nup153 antigen."

I seems therefore that both bands might correspond to nup153. Have you tried an alternative gel-buffer system to see whether you have obtained only one band in that case?

I have also noticed that nup153 is a highly phosphorylated protein. It could be that one of the two bands might be a phospho-form? Maybe an incubation with phosphatases would elucidate this point? Are you using phosphatase inhibitors in your samples?

Please do let us know your comments on this. If you would like us to continue to investigate this issue, I would be please to do so. In that case, could you please send us the protocol used and an image? I have attached a questionnaire which would help you to put the protocol information together.

I am looking forward to hear back from you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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