Anti-Nup153 antibody [QE5] (ab24700)
Key features and details
- Mouse monoclonal [QE5] to Nup153
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-Nup153 antibody [QE5]
See all Nup153 primary antibodies -
Description
Mouse monoclonal [QE5] to Nup153 -
Host species
Mouse -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF MouseHuman -
Immunogen
Full length protein corresponding to Rat Nup153.
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Positive control
- In Immunocytochemistry, this antibody gave a positive signal in HepG2 cells.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
QE5 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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ICC/IF |
Mouse
Human
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Application | Abreviews | Notes |
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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Notes |
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
Sequence similarities
Contains 4 RanBP2-type zinc fingers. -
Domain
Contains F-X-F-G repeats. -
Cellular localization
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
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Database links
- Entrez Gene: 9972 Human
- Entrez Gene: 218210 Mouse
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
- Unigene: 255398 Mouse
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Alternative names
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (ab24700)This image is courtesy of an anonymous Abreview.
Immunocytochemical analysis of U2OS cells, labeling Nup153 with ab24700. Cells were formaldehyde fixed, permeabilized with NP40, and blocked with 3% BSA for 1 hour at 21°C. Immunostaining with ab24700 diluted 1/100 for 12 hours at 4°C.
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Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (ab24700)This image is courtesy of Patrizia Lavia & Maria de Luca, University La Sapienza
Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 4% formaldehyde and permeabilized in 0.2% Triton x100 for 10 minutes at room temperature, then washed 3 times in PBS. The fixation and permeabilization was carried out in a single step. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).
A: HeLa cells, ab24700 used at 4µg/ml
B: 3T3 cells, ab24700 used at 2µg/ml
C: 3T3 cells, ab24700 used at 4µg/ml
D: 3T3 cells, ab24700 used at 6.6µg/ml -
Overlay histogram showing HeLa cells stained with ab24700 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24700, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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ICC/IF image of ab24700 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24700, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Methanol fixed HeLa stained with ab24700. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with Giantin (yellow).
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Immunocytochemistry/ Immunofluorescence - Anti-Nup153 antibody [QE5] (ab24700)This image is courtesy of Patrizia Lavia & Maria de Luca, University La Sapienza
Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 100% methanol for 20 minutes at -20°C, then washed once in PBS. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).
A: HeLa cells, ab24700 used at 4µg/ml
B: 3T3 cells, ab24700 used at 2µg/ml
C: 3T3 cells, ab24700 used at 4µg/ml
D: 3T3 cells, ab24700 used at 6.6µg/ml
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab24700 has been referenced in 44 publications.
- Bejarano DA et al. HIV-1 nuclear import in macrophages is regulated by CPSF6-capsid interactions at the nuclear pore complex. Elife 8:N/A (2019). PubMed: 30672737
- Han M et al. Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation. Hum Mutat 40:310-325 (2019). PubMed: 30488537
- Bikkul MU et al. Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform. Genes Chromosomes Cancer 58:341-356 (2019). PubMed: 30474255
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450
- de Castro IJ et al. CDK1 and PLK1 coordinate the disassembly and reassembly of the nuclear envelope in vertebrate mitosis. Oncotarget 9:7763-7773 (2018). PubMed: 29487689