• Product name

    Anti-Nup153 antibody [R4C8]
    See all Nup153 primary antibodies
  • Description

    Rat monoclonal [R4C8] to Nup153
  • Host species

  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Recombinant fragment, corresponding to amino acids 610-1191 of Rat Nup153, fused to a tag.

  • Epitope

    Amino acids 610-1191 (Zn finger and FG repeats domain).
  • Positive control

    • WB: Nuclear membrane fraction of HeLa cells and NIH3T3 cells. ICC/IF: HeLa cells and rat neuron.



Our Abpromise guarantee covers the use of ab81463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 154 kDa.
ICC/IF Use at an assay dependent dilution.



  • All lanes : Anti-Nup153 antibody [R4C8] (ab81463)

    Lane 1 : Nuclear membrane fraction from HeLa cells
    Lane 2 : Nuclear membrane fraction from NIH3T3 cells

    Predicted band size: 154 kDa
    Observed band size: 160 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent staining of HeLa cells with ab81463.
  • Immunofluorescent staining of rat neuron with ab81463. The dots are Nup153.


ab81463 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


Thank you for confirming that information.
I have processed your request to receive ab81463 as ab93310 did not work in western blot.
If there is anything else I can help you with, please let me know.

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Thank you for that,
I believe I have found the original order and so I just wanted to confirm the shipping date (just to make sure). Was it ordered on Jan 16th 2012, or about that date? If for any reason you need to contact us about a refund or replacement, it would be very helpful if you could let us know that you placed the order through one of our sub-dealers. That way I or my colleagues will be able to locate your order faster.
Once again, I look forward to your reply.

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Thank you for sending that information, unfortunately I cannot find you order with that information. Did you order it directly from Abcam, or did you order it through a third party?
I will continue to search, but could you let me know who purchased the antibody and the rough date of when it was purchased.
Thank you for your patience and I look forward to your response.

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Thank you for providing that information and sorry for the confusion about the antibody in question.
I agree that the conditions and quantities you are using should be more than sufficient to get a signal in western blot using ab93310. Therefore I would be happy to replace the antibody for you with ab81463.
If you could provide me with some of the original order information so that I can have this replacement sent to you as soon as possible that would be great.
I look forward to your reply and getting you the new antibody.

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Thank you for your reply.
I am sorry that you are having problems with ab81463 in both western blot and IP, they are covered under our Abpromise to work in both these applications.
However, could you please provide me some more information about the WB protocol that you used:
1 - What sample type are you using?
2 - How much sample are you loading?
3 - What type of blocking agent are you using?
4 - What primary antibody concentrations have you tried and how long do you incubate for?
I appreciate you providing this information and if I cannot help identify the problem, then I would be happy to send you a replacement antibody.
I look forward to your reply and helping resolve this problem.

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Thank you for contacting Abcam.
I am sorry that you were having trouble opening the webpage for ab81463, please try the link below:
I have also attached the datasheet for this product so that you can look through that.
Please let me know if you continue to have problems with this, or if there is anything else I can help you with.

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