Anti-Nup153 antibody [R4C8] (ab81463)
Key features and details
- Rat monoclonal [R4C8] to Nup153
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Nup153 antibody [R4C8]
See all Nup153 primary antibodies -
Description
Rat monoclonal [R4C8] to Nup153 -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to amino acids 610-1191 of Rat Nup153, fused to a tag.
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Epitope
Amino acids 610-1191 (Zn finger and FG repeats domain). -
Positive control
- WB: Nuclear membrane fraction of HeLa cells and NIH3T3 cells. ICC/IF: HeLa cells and rat neuron.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
This antibody was purified from the serum free cultured medium of the hybridoma under mild conditions by propriety chromatography processes. It is filter sterilized. -
Clonality
Monoclonal -
Clone number
R4C8 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 154 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
Sequence similarities
Contains 4 RanBP2-type zinc fingers. -
Domain
Contains F-X-F-G repeats. -
Cellular localization
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
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Database links
- Entrez Gene: 9972 Human
- Entrez Gene: 218210 Mouse
- Entrez Gene: 25281 Rat
- Omim: 603948 Human
- SwissProt: P49790 Human
- SwissProt: P49791 Rat
- Unigene: 601591 Human
- Unigene: 718703 Human
see all -
Alternative names
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
Images
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All lanes : Anti-Nup153 antibody [R4C8] (ab81463)
Lane 1 : Nuclear membrane fraction from HeLa cells
Lane 2 : Nuclear membrane fraction from NIH3T3 cells
Predicted band size: 154 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted? -
Immunofluorescent staining of HeLa cells with ab81463.
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Immunofluorescent staining of rat neuron with ab81463. The dots are Nup153.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab81463 has been referenced in 2 publications.
- Uhlírová J et al. Nucleoporin TPR Affects C2C12 Myogenic Differentiation via Regulation of Myh4 Expression. Cells 10:N/A (2021). PubMed: 34063931
- Tran JR et al. An APEX2 proximity ligation method for mapping interactions with the nuclear lamina. J Cell Biol 220:N/A (2021). PubMed: 33306092