Key features and details
- Mouse monoclonal [SA1] to Nup153
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig
- Isotype: IgG
Product nameAnti-Nup153 antibody [SA1]
See all Nup153 primary antibodies
DescriptionMouse monoclonal [SA1] to Nup153
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Dog, Human, Pig
corresponding to Nup153.
- This antibody gave a positive signal in HepG2 cells (Immunocytochemistry). This antibody gave a positive result in IHC in the following FFPE tissue: Human breast adenocarcinoma.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Our Abpromise guarantee covers the use of ab96462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionPossible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane.
Sequence similaritiesContains 4 RanBP2-type zinc fingers.
DomainContains F-X-F-G repeats.
Cellular localizationNucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage.
- Information by UniProt
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
ICC/IF image of ab96462 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96462, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.
IHC image of Nup153 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96462, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab96462 has been referenced in 7 publications.
- Pappas SS et al. TorsinA dysfunction causes persistent neuronal nuclear pore defects. Hum Mol Genet 27:407-420 (2018). PubMed: 29186574
- Lång A et al. Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites. Nucleus 8:404-420 (2017). PubMed: 28402725
- Pérez-Garrastachu M et al. Nucleoporins redistribute inside the nucleus after cell cycle arrest induced by histone deacetylases inhibition. Nucleus 8:515-533 (2017). PubMed: 28696859
- Lowe AR et al. Importin-ß modulates the permeability of the nuclear pore complex in a Ran-dependent manner. Elife 4:N/A (2015). PubMed: 25748139
- Schooley A et al. The lysine demethylase LSD1 is required for nuclear envelope formation at the end of mitosis. J Cell Sci 128:3466-77 (2015). PubMed: 26224877
- Rodriguez AC et al. Enhanced transfer of a photocross-linking N-acetylglucosamine (GlcNAc) analog by an O-GlcNAc transferase mutant with converted substrate specificity. J Biol Chem 290:22638-48 (2015). PubMed: 26240142
- Walker EJ et al. Rhinovirus 3C protease facilitates specific nucleoporin cleavage and mislocalisation of nuclear proteins in infected host cells. PLoS One 8:e71316 (2013). WB, IF . PubMed: 23951130