Key features and details
- Rabbit polyclonal to NUP155
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NUP155 antibody
See all NUP155 primary antibodies
DescriptionRabbit polyclonal to NUP155
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
- 293T, HeLa, Jurkat and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157104 was affinity purified using an epitope specific to NUP155 immobilized on solid support.
Our Abpromise guarantee covers the use of ab157104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 155 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEssential component of nuclear pore complex. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.
Tissue specificityExpressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the non-repetitive/WGA-negative nucleoporin family.
modificationsPhosphorylated. Phosphorylation and dephosphorylation may be important for the function of NUP155 and may play a role in the reversible disassembly of the nuclear pore complex during mitosis.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Nucleus membrane. In mitosis, assumes a diffuse cytoplasmic distribution probably as a monomer, before reversing back into a punctate nuclear surface localization at the end of mitosis.
- Information by UniProt
- 155 kDa nucleoporin antibody
- ATFB15 antibody
- D930027M19Rik antibody
All lanes : Anti-NUP155 antibody (ab157104) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 155 kDa
Exposure time: 3 minutes
Detection of NUP155 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab157104 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab157104 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab157104 has not yet been referenced specifically in any publications.