Key features and details
- Rabbit polyclonal to NUP155
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUP155 antibody
See all NUP155 primary antibodies
DescriptionRabbit polyclonal to NUP155
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment (His-T7-tag) corresponding to Human NUP155 aa 1154-1379. (Expressed in E.coli).
QLQIQETLQRQYSHHSSVQDAVSQLDSELMDITKLYGEFADPFKLAECKL AIIHCAGYSDPILVQTLWQDIIEKELSDSVTLSSSDRMHALSLKIVLLGK IYAGTPRFFPLDFIVQFLEQQVCTLNWDVGFVIQTMNEIGVPLPRLLEVY DQLFKSRDPFWNRMKKPLHLLDCIHVLLIRYVENPSQVLNCERRRFTNLC LDAVCGYLVELQSMSSSVAVQAITGN
Database link: O75694
- WB: Recombinant human NUP155 protein; HEK-293T, HeLa and Jurkat cell lysates. IHC-P: Human prostate, glioma, kidney and colon tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab233079 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 155 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionEssential component of nuclear pore complex. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.
Tissue specificityExpressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the non-repetitive/WGA-negative nucleoporin family.
modificationsPhosphorylated. Phosphorylation and dephosphorylation may be important for the function of NUP155 and may play a role in the reversible disassembly of the nuclear pore complex during mitosis.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Nucleus membrane. In mitosis, assumes a diffuse cytoplasmic distribution probably as a monomer, before reversing back into a punctate nuclear surface localization at the end of mitosis.
- Information by UniProt
- 155 kDa nucleoporin antibody
- ATFB15 antibody
- D930027M19Rik antibody
All lanes : Anti-NUP155 antibody (ab233079) at 3 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 155 kDa
Anti-NUP155 antibody (ab233079) at 3 µg/ml + Recombinant human NUP155 protein.
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 155 kDa
Paraffin-embedded human colon tissue stained for NUP155 using ab233079 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for NUP155 using ab233079 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human glioma tissue stained for NUP155 using ab233079 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate tissue stained for NUP155 using ab233079 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233079 has not yet been referenced specifically in any publications.