Overview

  • Product name

    Anti-NUP155 antibody [EPR17111]
    See all NUP155 primary antibodies
  • Description

    Rabbit monoclonal [EPR17111] to NUP155
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NUP155 aa 900-1050. The exact sequence is proprietary.
    Database link: O75694

  • Positive control

    • WB: Jurkat, HEK293 and HeLa cells lysates; Human fetal liver tissue lysate. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
Flow Cyt 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/2000.

Target

  • Function

    Essential component of nuclear pore complex. Nucleoporins may be involved both in binding and translocating proteins during nucleocytoplasmic transport.
  • Tissue specificity

    Expressed in all tissues tested, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the non-repetitive/WGA-negative nucleoporin family.
  • Post-translational
    modifications

    Phosphorylated. Phosphorylation and dephosphorylation may be important for the function of NUP155 and may play a role in the reversible disassembly of the nuclear pore complex during mitosis.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Nucleus membrane. In mitosis, assumes a diffuse cytoplasmic distribution probably as a monomer, before reversing back into a punctate nuclear surface localization at the end of mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • 155 kDa nucleoporin antibody
    • ATFB15 antibody
    • D930027M19Rik antibody
    • KIAA0791 antibody
    • mKIAA0791 antibody
    • N155 antibody
    • NU155_HUMAN antibody
    • Nuclear pore complex protein Nup155 antibody
    • nucleoporin 155kD antibody
    • Nucleoporin Nup155 antibody
    • Nup155 antibody
    see all

Images

  • All lanes : Anti-NUP155 antibody [EPR17111] (ab199528) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 155 kDa
    Observed band size: 155 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUP155 with ab199528 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nucleus membrane and cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab199528 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUP155 with ab199528 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-NUP155 antibody [EPR17111] (ab199528) at 1/2000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 155 kDa
    Observed band size: 155 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-NUP155 antibody [EPR17111] (ab199528) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 155 kDa
    Observed band size: 155 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Han M  et al. Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation. Hum Mutat 40:310-325 (2019). Read more (PubMed: 30488537) »
  • Lång A  et al. Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites. Nucleus 8:404-420 (2017). Read more (PubMed: 28402725) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
28 cells
Specification
fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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Verified customer

Submitted Jun 06 2019

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