Overview

  • Product name

    Anti-NUP160 antibody
  • Description

    Rabbit polyclonal to NUP160
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human NUP160 (C terminal).
    Database link: EAW67878.1

  • Positive control

    • Rat brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 149 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

Target

Images

  • Lane 1 : Anti-NUP160 antibody (ab73293) at 0.5 µg/ml
    Lane 2 : Anti-NUP160 antibody (ab73293) at 1 µg/ml

    All lanes : rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 149 kDa
    Observed band size: 160,70 kDa
    why is the actual band size different from the predicted?

  • ab73293 at 2.5µg/ml staining NUP160 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of NUP160 in Human Brain cells using ab73293 at 20 ug/ml.

References

ab73293 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. Unfortunately, the exact immunogen sequence for this product is proprietary and cannot be released. However, I ran a BLAST with zebrafish and found 50% homology with NP_001073512.1 and 43.7% with sorting nexin 5 and sorting nexin 6. Based on these percentages, I would assume that the antibody will probably not work for zebrafish. If there is anything else that I can help you with, please do not hesitate to contact me.

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