Overview

  • Product name

  • Description

    Rabbit polyclonal to NUP188
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1699 and 1749 of human NUP188 (NP_056169.1)

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 196 kDa (predicted molecular weight: 196 kDa).
IP Use at 10 µg/mg of lysate.

Target

  • Relevance

    NUP188 may function as a component of the nuclear pore complex (NPC).
  • Cellular localization

    Nucleus; nuclear pore complex
  • Database links

  • Alternative names

    • BC025526 antibody
    • FLJ21639 antibody
    • hNup188 antibody
    • KIAA0169 antibody
    • mKIAA0169 antibody
    • Nucleoporin 188kDa antibody
    • Nucleoporin NUP188 homolog antibody
    • OTTMUSP00000018197 antibody
    • RP11-167N5.2 antibody
    • RP23-395P6.3 antibody
    • U89435 antibody
    see all

Images

  • All lanes : Anti-NUP188 antibody (ab86601) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 196 kDa
    Observed band size: 196 kDa


    Exposure time: 3 minutes
  • Detection of NUP188 by Western Blot of Immunprecipitate.
    ab86601 at 1µg/ml staining NUP188 in HeLa whole cell lysate immunoprecipitated using ab86601 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Labade AS  et al. HOXA repression is mediated by nucleoporin Nup93 assisted by its interactors Nup188 and Nup205. Epigenetics Chromatin 9:54 (2016). WB ; Human . Read more (PubMed: 27980680) »
  • Ladopoulos V  et al. The Histone Methyltransferase KMT2B Is Required for RNA Polymerase II Association and Protection from DNA Methylation at the MagohB CpG Island Promoter. Mol Cell Biol 33:1383-93 (2013). Read more (PubMed: 23358417) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 22 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Specification
HeLa
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 20 2019

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Colorectal cancer cell line DLD1)
Total protein in input
500 µg
Immuno-precipitation step
Other - Protein A Dynabeads
Specification
Colorectal cancer cell line DLD1

Ajay Labade

Verified customer

Submitted Jan 31 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (DLD1 cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
DLD1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ajay Labade

Verified customer

Submitted Jan 30 2017

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