Overview

  • Product name

    Anti-NUP205 antibody
  • Description

    Rabbit polyclonal to NUP205
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1750-1800 of Human NUP205 (NP_055950.1).

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab157090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 228 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-NUP205 antibody (ab157090) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 228 kDa


    Exposure time: 3 minutes
  • ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes NUP205
    Lane 2: ab157090 at 6 µg/mg of lysate
    Lane 3: Control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab157090 has not yet been referenced specifically in any publications.

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