Key features and details
- Rabbit polyclonal to NUP205
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUP205 antibody
DescriptionRabbit polyclonal to NUP205
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region within amino acids 1750-1800 of Human NUP205 (NP_055950.1).
- 293T, HeLa and Jurkat whole cell lysate (ab7899)
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support.
Our Abpromise guarantee covers the use of ab157090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 228 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Cellular localizationNucleus > nuclear pore complex.
- Information by UniProt
- 205 kDa nucleoporin antibody
- C7orf14 antibody
- KIAA0225 antibody
All lanes : Anti-NUP205 antibody (ab157090) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 228 kDa
Exposure time: 3 minutes
ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes NUP205
Lane 2: ab157090 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab157090 has been referenced in 1 publication.
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143