Product nameAnti-NUP214 antibody
See all NUP214 primary antibodies
DescriptionRabbit polyclonal to NUP214
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human NUP214 aa 2050-2090 (C terminal). The exact sequence is proprietary.
Database link: P35658
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
Tissue specificityExpressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
Involvement in diseaseNote=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
DomainContains FG repeats.
modificationsProbably glycosylated as it reacts with wheat germ agglutinin (WGA).
Cellular localizationNucleus > nuclear pore complex. Cytoplasmic filaments.
- Information by UniProt
- 214 kDa nucleoporin antibody
- CAIN antibody
- CAN antibody
All lanes : Anti-NUP214 antibody (ab264143) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Exposure time: 3 minutes
NUP214 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264143 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264143 at 1 µg/ml.
Lane 1: ab264143 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264143 has not yet been referenced specifically in any publications.