Key features and details
- Rabbit polyclonal to NUP214
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NUP214 antibody
See all NUP214 primary antibodies
DescriptionRabbit polyclonal to NUP214
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1250 and 1300 of human NUP214 (NP_005076.3).
- Human ovarian carcinoma. NBF-fixed asynchronous HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/100 - 1/500. Fix with formaldehyde.|
FunctionMay serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.
Tissue specificityExpressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.
Involvement in diseaseNote=A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene.
Note=A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET.
DomainContains FG repeats.
modificationsProbably glycosylated as it reacts with wheat germ agglutinin (WGA).
Cellular localizationNucleus > nuclear pore complex. Cytoplasmic filaments.
- Information by UniProt
- 214 kDa nucleoporin antibody
- CAIN antibody
- CAN antibody
ab84357, at 1/100 dilution, staining NUP214 in formalin-fixed, paraffin-embedded human ovarian carcinoma tissue by Immunohistochemistry using DAB detection and an anti-Rabbit IgG HRP conjugated secondary antibody at a dilution of 1/100.
ab84357, at 1/250 dilution, staining NUP214 in NBF-fixed asynchronous HeLa cells by Immunofluorescence using an Anti-rabbit IgG-FITC conjugated secondary antibody at a dilution of 1/100.
ab84357 has been referenced in 2 publications.
- Schwartz M et al. Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin. Nucleus 6:40-54 (2015). PubMed: 25602437
- Di Nunzio F et al. Human Nucleoporins Promote HIV-1 Docking at the Nuclear Pore, Nuclear Import and Integration. PLoS One 7:e46037 (2012). WB, ICC/IF . PubMed: 23049930