Anti-NUP35 antibody (ab11692)
Key features and details
- Rabbit polyclonal to NUP35
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-NUP35 antibody
See all NUP35 primary antibodies -
Description
Rabbit polyclonal to NUP35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Fusion protein corresponding to Saccharomyces cerevisiae NUP35.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 53 kDa.
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ICC/IF |
Use at an assay dependent dilution.
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Notes |
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WB
1/1000. Detects a band of approximately 53 kDa. |
ICC/IF
Use at an assay dependent dilution. |
Target
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Function
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs). Can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC. -
Sequence similarities
Belongs to the Nup53 family.
Contains 1 RRM Nup35-type domain. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Tightly associated with the nuclear membrane and lamina. - Information by UniProt
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Alternative names
- 35 kDa nucleoporin antibody
- Mitotic phosphoprotein 44 antibody
- MP 44 antibody
see all
Images
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ab11692 at a 1/1000 dilution staining approximately 53kDa Nup35 in S.cerevisiae nuclear and whole cell lysate by Western blot (ECL). ab11692 at a 1/1000 dilution staining approximately 53kDa Nup35 in S.cerevisiae nuclear and whole cell lysate by Western blot (ECL).
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ab11692 at a 1/1000 dilution staining Nup35 in S.cerevisiae by immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab11692 has been referenced in 1 publication.
- Guet D et al. Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast. Nat Commun 6:8882 (2015). PubMed: 26582123