Anti-NUP43 antibody (ab235420)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NUP43 antibody
See all NUP43 primary antibodies -
Description
Rabbit polyclonal to NUP43 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NUP43 aa 1-200.
Database link: Q8NFH3 -
Positive control
- WB: A431, HL-60, THP-1 and A549 whole cell lysates. IHC-P: Human testis tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab235420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 42 kDa. | |
| IHC-P | 1/20 - 1/200. |
Target
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Relevance
NUP43 probably mediates the assembly of subdomains of the nuclear pore complex (NPC) or facilitates the interaction of transport complexes with the NPC. -
Cellular localization
Nucleus; nuclear pore complex. -
Database links
- Entrez Gene: 348995 Human
- Omim: 608141 Human
- SwissProt: Q8NFH3 Human
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Alternative names
- bA350J20.1 antibody
- FLJ13287 antibody
- Nucleoporin 43kDa antibody
see all
Images
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Western blot - Anti-NUP43 antibody (ab235420)All lanes : Anti-NUP43 antibody (ab235420) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 42 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP43 antibody (ab235420)Paraffin-embedded human testis tissue stained for NUP43 using ab235420 at 1/100 dilution in immunohistochemical analysis.
References
ab235420 has not yet been referenced specifically in any publications.
