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RecombinantRabMAb

Recombinant Anti-NUP50 antibody [EPR9525] (ab151567)

Submit a review Submit a question References (2)
Western blot - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
  • Anti-NUP50 antibody [EPR9525] (ab151567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9525] to NUP50
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-NUP50 antibody [EPR9525]
    See all NUP50 primary antibodies

  • Description

    Rabbit monoclonal [EPR9525] to NUP50

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human NUP50 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa, 293T and HepG2 cell lysates; Human colon tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.

  • Tissue specificity

    Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.

  • Sequence similarities

    Contains 1 RanBD1 domain.

  • Domain

    Contains FG repeats.

  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 50 kDa nucleoporin antibody
    • NPAP60 antibody
    • NPAP60L antibody
    • Nuclear pore associated protein 60L antibody
    • Nuclear pore complex protein Nup50 antibody
    • Nuclear pore-associated protein 60 kDa-like antibody
    • Nucleoporin 50 antibody
    • Nucleoporin 50kDa antibody
    • Nucleoporin Nup50 antibody
    • Nup50 antibody
    • NUP50_HUMAN antibody
    • PRO1146 antibody
    see all

Images

  • Western blot - Anti-NUP50 antibody [EPR9525] (ab151567)
    Western blot - Anti-NUP50 antibody [EPR9525] (ab151567)
    All lanes : Anti-NUP50 antibody [EPR9525] (ab151567) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUP50 with ab151567 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunofluorescent analysis of HeLa cells labeling NUP50 with ab151567 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151567 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

    Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151567 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

    Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151567 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

    Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151567 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151567 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-NUP50 antibody [EPR9525] (ab151567)
    Anti-NUP50 antibody [EPR9525] (ab151567)

References (2)

Publishing research using ab151567? Please let us know so that we can cite the reference in this datasheet.

ab151567 has been referenced in 2 publications.

  • Jacinto FV  et al. The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes Dev 29:1224-38 (2015). PubMed: 26080816
  • Buchwalter AL  et al. Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics. Mol Biol Cell 25:2472-84 (2014). PubMed: 24943837

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