Recombinant Anti-NUP50 antibody [EPR9525] (ab151567)

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Western blot - Anti-NUP50 antibody [EPR9525] (ab151567)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9525] to NUP50
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Carrier Free

Product name

Anti-NUP50 antibody [EPR9525]
See all NUP50 primary antibodies
Description

Rabbit monoclonal [EPR9525] to NUP50
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide within Human NUP50 aa 1-100. The exact sequence is proprietary.
Positive control
- Jurkat, HeLa, 293T and HepG2 cell lysates; Human colon tissue; HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9525
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Other
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Other

Alternative Versions
- Anti-NUP50 antibody [EPR9525] - BSA and Azide free (ab240121)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab7899)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt.

Function

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
Tissue specificity

Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
Sequence similarities

Contains 1 RanBD1 domain.
Domain

Contains FG repeats.
Cellular localization

Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
Target information above from: UniProt accession Q9UKX7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 10762 Human
- Entrez Gene: 18141 Mouse
- Entrez Gene: 25497 Rat
- Omim: 604646 Human
- SwissProt: Q9UKX7 Human
- SwissProt: Q9JIH2 Mouse
- SwissProt: O08587 Rat
- Unigene: 727643 Human
- Unigene: 28379 Mouse
- Unigene: 391486 Mouse
- Unigene: 123156 Rat
- Unigene: 3242 Rat
see all
Alternative names
- 50 kDa nucleoporin antibody
- NPAP60 antibody
- NPAP60L antibody
- Nuclear pore associated protein 60L antibody
- Nuclear pore complex protein Nup50 antibody
- Nuclear pore-associated protein 60 kDa-like antibody
- Nucleoporin 50 antibody
- Nucleoporin 50kDa antibody
- Nucleoporin Nup50 antibody
- Nup50 antibody
- NUP50_HUMAN antibody
- PRO1146 antibody
see all

Western blot - Anti-NUP50 antibody [EPR9525] (ab151567)

All lanes : Anti-NUP50 antibody [EPR9525] (ab151567) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUP50 with ab151567 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunofluorescent analysis of HeLa cells labeling NUP50 with ab151567 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151567 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-NUP50 antibody [EPR9525] (ab151567)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab151567? Please let us know so that we can cite the reference in this datasheet.

ab151567 has been referenced in 2 publications.

Jacinto FV et al. The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing. Genes Dev 29:1224-38 (2015). PubMed: 26080816
Buchwalter AL et al. Nup50 is required for cell differentiation and exhibits transcription-dependent dynamics. Mol Biol Cell 25:2472-84 (2014). PubMed: 24943837

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

References (2)

Customer reviews and Q&As