Recombinant
RabMAb

Anti-NUP50 antibody [EPR9525] - BSA and Azide free (ab240121)

Overview

  • Product name
    Anti-NUP50 antibody [EPR9525] - BSA and Azide free
    See all NUP50 primary antibodies
  • Description
    Rabbit monoclonal [EPR9525] to NUP50 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NUP50 aa 1-100. The exact sequence is proprietary.

  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab240121 is a PBS-only buffer format of ab151567. Please refer to ab151567 for recommended dilutions, protocols, and image data.

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
    • Tissue specificity
      Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
    • Sequence similarities
      Contains 1 RanBD1 domain.
    • Domain
      Contains FG repeats.
    • Cellular localization
      Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • 50 kDa nucleoporin antibody
      • NPAP60 antibody
      • NPAP60L antibody
      • Nuclear pore associated protein 60L antibody
      • Nuclear pore complex protein Nup50 antibody
      • Nuclear pore-associated protein 60 kDa-like antibody
      • Nucleoporin 50 antibody
      • Nucleoporin 50kDa antibody
      • Nucleoporin Nup50 antibody
      • Nup50 antibody
      • NUP50_HUMAN antibody
      • PRO1146 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NUP50 with ab151567 at 1/50 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    • Immunofluorescent analysis of HeLa cells labeling NUP50 with ab151567 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab151567 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab151567 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    • Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151567 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    • Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151567 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151567 showing +ve staining.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab151567).

    References

    ab240121 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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