Recombinant Anti-NUP50 antibody [EPR9526] (ab137092)
![Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151676-anti-nup50-antibody-epr9526-western-blot.jpg "Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9526] to NUP50
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NUP50 antibody [EPR9526]
See all NUP50 primary antibodies -
Description
Rabbit monoclonal [EPR9526] to NUP50 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human NUP50 aa 300-400. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa and 293T cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9526 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
|
|
| ICC/IF | (1) |
1/250 - 1/500.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
|
ICC/IF
1/250 - 1/500. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. -
Tissue specificity
Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. -
Sequence similarities
Contains 1 RanBD1 domain. -
Domain
Contains FG repeats. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex. - Information by UniProt
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Database links
- Entrez Gene: 10762 Human
- Entrez Gene: 18141 Mouse
- Entrez Gene: 25497 Rat
- Omim: 604646 Human
- SwissProt: Q9UKX7 Human
- SwissProt: Q9JIH2 Mouse
- SwissProt: O08587 Rat
- Unigene: 727643 Human
see all -
Alternative names
- 50 kDa nucleoporin antibody
- NPAP60 antibody
- NPAP60L antibody
see all
Images
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Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)All lanes : Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151675-anti-nup50-antibody-epr9526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151674-anti-nup50-antibody-epr9526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)This image is courtesy of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview. -
![Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-4-benefits-of-recombinant-antibodies.png)
Anti-NUP50 antibody [EPR9526] (ab137092)
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab137092 has been referenced in 2 publications.
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143
- Linder MI et al. Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins. Dev Cell 43:141-156.e7 (2017). PubMed: 29065306
