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    nup50-antibody-epr9526-ab137092.pdf

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RecombinantRabMAb

Recombinant Anti-NUP50 antibody [EPR9526] (ab137092)

  • Datasheet
Reviews (1) Submit a question References (2)

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Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)
  • Anti-NUP50 antibody [EPR9526] (ab137092)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9526] to NUP50
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-NUP50 antibody [EPR9526]
    See all NUP50 primary antibodies
  • Description

    Rabbit monoclonal [EPR9526] to NUP50
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human NUP50 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa and 293T cell lysates; HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9526
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
    • Signal Transduction
    • Protein Trafficking
    • Nuclear Import / Export
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-NUP50 antibody [EPR9526] (ab208551)
    • Anti-NUP50 antibody [EPR9526] - BSA and Azide free (ab240104)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF (1)
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
  • Tissue specificity

    Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
  • Sequence similarities

    Contains 1 RanBD1 domain.
  • Domain

    Contains FG repeats.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
  • Target information above from: UniProt accession Q9UKX7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10762 Human
    • Entrez Gene: 18141 Mouse
    • Entrez Gene: 25497 Rat
    • Omim: 604646 Human
    • SwissProt: Q9UKX7 Human
    • SwissProt: Q9JIH2 Mouse
    • SwissProt: O08587 Rat
    • Unigene: 727643 Human
    • Unigene: 28379 Mouse
    • Unigene: 391486 Mouse
    • Unigene: 123156 Rat
    • Unigene: 3242 Rat
    see all
  • Alternative names

    • 50 kDa nucleoporin antibody
    • NPAP60 antibody
    • NPAP60L antibody
    • Nuclear pore associated protein 60L antibody
    • Nuclear pore complex protein Nup50 antibody
    • Nuclear pore-associated protein 60 kDa-like antibody
    • Nucleoporin 50 antibody
    • Nucleoporin 50kDa antibody
    • Nucleoporin Nup50 antibody
    • Nup50 antibody
    • NUP50_HUMAN antibody
    • PRO1146 antibody
    see all

Images

  • Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)
    Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)
    All lanes : Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)
    Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)This image is courtesy of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
    ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • Anti-NUP50 antibody [EPR9526] (ab137092)
    Anti-NUP50 antibody [EPR9526] (ab137092)

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab137092? Please let us know so that we can cite the reference in this datasheet.

ab137092 has been referenced in 2 publications.

  • Zhang K  et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143
  • Linder MI  et al. Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins. Dev Cell 43:141-156.e7 (2017). PubMed: 29065306

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunocytochemistry/ Immunofluorescence abreview for Anti-NUP50 antibody [EPR9526]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Nov 19 2012

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