Recombinant
Recombinant Anti-NUP50 antibody [EPR9526] (ab137092)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-NUP50 antibody [EPR9526]
See all NUP50 primary antibodies -
Description
Rabbit monoclonal [EPR9526] to NUP50 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NUP50 aa 300-400. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa and 293T cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9526 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab137092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 50 kDa. | |
| ICC/IF | 1/250 - 1/500. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. -
Tissue specificity
Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. -
Sequence similarities
Contains 1 RanBD1 domain. -
Domain
Contains FG repeats. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex. - Information by UniProt
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Database links
- Entrez Gene: 10762 Human
- Entrez Gene: 18141 Mouse
- Entrez Gene: 25497 Rat
- Omim: 604646 Human
- SwissProt: Q9UKX7 Human
- SwissProt: Q9JIH2 Mouse
- SwissProt: O08587 Rat
- Unigene: 727643 Human
see all -
Alternative names
- 50 kDa nucleoporin antibody
- NPAP60 antibody
- NPAP60L antibody
see all
Images
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![Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151676-anti-nup50-antibody-epr9526-western-blot.jpg)
Western blot - Anti-NUP50 antibody [EPR9526] (ab137092)All lanes : Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 50 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151675-anti-nup50-antibody-epr9526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)](https://www.abcam.com/ps/products/137/ab137092/Images/ab137092-151674-anti-nup50-antibody-epr9526-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP50 antibody [EPR9526] (ab137092)This image is courtesy of an abreview submitted by Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). Read more (PubMed: 29628143) »
- Linder MI et al. Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins. Dev Cell 43:141-156.e7 (2017). Read more (PubMed: 29065306) »
