Overview

  • Product name
    Anti-NUP50 antibody [EPR9526]
    See all NUP50 primary antibodies
  • Description
    Rabbit monoclonal [EPR9526] to NUP50
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NUP50 aa 300-400. The exact sequence is proprietary.

  • Positive control
    • Jurkat, HeLa and 293T cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
    • Tissue specificity
      Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
    • Sequence similarities
      Contains 1 RanBD1 domain.
    • Domain
      Contains FG repeats.
    • Cellular localization
      Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
    • Information by UniProt
    • Database links
    • Alternative names
      • 50 kDa nucleoporin antibody
      • NPAP60 antibody
      • NPAP60L antibody
      • Nuclear pore associated protein 60L antibody
      • Nuclear pore complex protein Nup50 antibody
      • Nuclear pore-associated protein 60 kDa-like antibody
      • Nucleoporin 50 antibody
      • Nucleoporin 50kDa antibody
      • Nucleoporin Nup50 antibody
      • Nup50 antibody
      • NUP50_HUMAN antibody
      • PRO1146 antibody
      see all

    Images

    • All lanes : Anti-NUP50 antibody [EPR9526] (ab137092) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 50 kDa

    • Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution.
    • ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    References

    This product has been referenced in:
    • Linder MI  et al. Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins. Dev Cell 43:141-156.e7 (2017). Read more (PubMed: 29065306) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Filter by Application

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Nov 19 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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