Recombinant Anti-NUP50 antibody [EPR9526] - BSA and Azide free (ab240104)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9526] to NUP50 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-NUP50 antibody [EPR9526] - BSA and Azide free
See all NUP50 primary antibodies -
Description
Rabbit monoclonal [EPR9526] to NUP50 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab240104 is the carrier-free version of ab137092.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9526 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. |
Target
-
Function
Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. -
Tissue specificity
Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. -
Sequence similarities
Contains 1 RanBD1 domain. -
Domain
Contains FG repeats. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex. - Information by UniProt
-
Database links
- Entrez Gene: 10762 Human
- Entrez Gene: 18141 Mouse
- Entrez Gene: 25497 Rat
- Omim: 604646 Human
- SwissProt: Q9UKX7 Human
- SwissProt: Q9JIH2 Mouse
- SwissProt: O08587 Rat
- Unigene: 727643 Human
see all -
Alternative names
- 50 kDa nucleoporin antibody
- NPAP60 antibody
- NPAP60L antibody
see all
Images
-
ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137092).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab240104 has not yet been referenced specifically in any publications.