Recombinant
RabMAb

Recombinant Anti-NUP50 antibody [EPR9526] - BSA and Azide free (ab240104)

Overview

  • Product name

    Anti-NUP50 antibody [EPR9526] - BSA and Azide free
    See all NUP50 primary antibodies
  • Description

    Rabbit monoclonal [EPR9526] to NUP50 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NUP50 aa 300-400. The exact sequence is proprietary.

  • General notes

    Ab240104 is the carrier-free version of ab137092. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240104 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.
    • Tissue specificity

      Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver.
    • Sequence similarities

      Contains 1 RanBD1 domain.
    • Domain

      Contains FG repeats.
    • Cellular localization

      Nucleus > nuclear pore complex. Nucleus membrane. Localizes to the nucleoplasmic fibrils of the nuclear pore complex.
    • Information by UniProt
    • Database links

    • Alternative names

      • 50 kDa nucleoporin antibody
      • NPAP60 antibody
      • NPAP60L antibody
      • Nuclear pore associated protein 60L antibody
      • Nuclear pore complex protein Nup50 antibody
      • Nuclear pore-associated protein 60 kDa-like antibody
      • Nucleoporin 50 antibody
      • Nucleoporin 50kDa antibody
      • Nucleoporin Nup50 antibody
      • Nup50 antibody
      • NUP50_HUMAN antibody
      • PRO1146 antibody
      see all

    Images

    • Immunofluorescent analysis of HeLa cells labelling NUP50 with ab137092 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137092).

    • ab137092 (1/200) staining NUP50 in HeLa Cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137092).

    References

    ab240104 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240104.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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