Overview

  • Product name

    Anti-NUP84 antibody
  • Description

    Goat polyclonal to NUP84
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide:

    ELSPTYQTERFTK-C

    , corresponding to N terminal amino acids 2-14 of Saccharomyces cerevisiae NUP84 according to NP_010167.1.

  • Positive control

    • Saccharomyces cerevisiae S288c lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 84 kDa).

Target

Images

  • All lanes : Anti-NUP84 antibody (ab122883) at 2 µg/ml (incubation time: 1 hour)

    Lane 1 : Saccharomyces cerevisiae S288c lysate in RIPA buffer - knock out
    Lane 2 : Saccharomyces cerevisiae S288c lysate in RIPA buffer - wild type

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 84 kDa

References

This product has been referenced in:

  • Chung DK  et al. Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process. Nat Commun 6:7742 (2015). Read more (PubMed: 26205667) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

As we have no comment regarding the incubation condition, it shouldn't be a problem to use your standard lab protocol and optimize the results from that point.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up