Key features and details
- Rabbit polyclonal to NUP88
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NUP88 antibody
See all NUP88 primary antibodies
DescriptionRabbit polyclonal to NUP88
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment (His-T7-tag) corresponding to Mouse NUP88 aa 57-296. Expressed in E.Coli. N-terminal tags.
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFLPTRNLVFGLGGEL FLWDAEGSAFLVVRLRGPSGGGVEPPLSQYQRLLCINPPLFEIHQVLLSP TQHHVALIGSKGLMALELPQRWGKDSEFEGGKATVNCSTIPIAERFFTSS TSLTLKHAAWYPSEMLDPHIVLLTSDNVIRIYSLREPQTPTKVIVLSEAE EESLILNKGRAYTASLGETAVAFDFGPLVTVSKNIFEQKDRDVVAYPLYI LYENGETFLTYVSLLHSPGNIGKLLG
Database link: Q5QNU0
- WB: Recombinant mouse NUP88 protein; mouse liver lysate; HeLa and HepG2 cell lysates. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 84 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionEssential component of nuclear pore complex.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex.
- Information by UniProt
- 88 kDa nucleoporin antibody
- karyoporin antibody
- Nuclear pore complex protein 88 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NUP88 (green) using ab231187 at 20 μg/ml in ICC/IF. FITC labeled secondary antibody.
All lanes : Anti-NUP88 antibody (ab231187) at 2 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa
Anti-NUP88 antibody (ab231187) at 2 µg/ml + Recombinant mouse NUP88 protein
Predicted band size: 84 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231187 has not yet been referenced specifically in any publications.