Overview

  • Product name
  • Description
    Rabbit polyclonal to NUP93
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Lizard, Snake, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human NUP93 aa 606-656. The exact sequence is proprietary.
    Database link: Q8N1F7

  • Positive control
    • 293T, HeLa and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 75 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    Part of the nucleoporin complex; required for correct nuclear pore assembly.
  • Cellular localization
    Nucleus, nuclear pore complex. Note: Localizes at the nuclear basket and at or near the nuclear entry to the gated channel of the pore.
  • Database links
  • Alternative names
    • 2410008G02Rik antibody
    • 93 kDa nucleoporin antibody
    • CBP-interacting protein 4 antibody
    • Dead eye protein antibody
    • Dye antibody
    • KIAA0095 antibody
    • MGC21106 antibody
    • NIC96 antibody
    • NIC96, S. cerevisiae, homolog of antibody
    • Nuclear pore complex protein Nup93 antibody
    • Nucleoporin 93 antibody
    • Nucleoporin 93kDa antibody
    • Nucleoporin Nup93 antibody
    see all

Images

  • All lanes : Anti-NUP93 antibody (ab168805) at 0.1 µg/ml

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 75 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. 1) A rabbit anti-NUP93 antibody immunoprecipitated NUP93. 2) ab168805 (6 µg/mg lysate). 3) control IgG. For blotting ab168805 was used at 1 µg/ml. Detection by chemiluminescence with an exposure time of 30 seconds.

References

ab168805 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (4-12% gel, MOPS soln)
Sample
Mouse Tissue lysate - whole (Intestine)
Specification
Intestine
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Sep 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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