Overview

  • Product name

    Anti-NUP98 antibody [13C2] - BSA and Azide free
    See all NUP98 primary antibodies
  • Description

    Mouse monoclonal [13C2] to NUP98 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
    Sequence:

    MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ


    Database link: D3KYQ3

    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664 (internal sequence).
    Sequence: SNPTQGGGLFGAANPGLGG
    Database link: D3KYQ3

Properties

Applications

Our Abpromise guarantee covers the use of ab179894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 112 kDa.
ICC/IF Use a concentration of 0.5 µg/ml.

Target

  • Function

    Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
  • Involvement in disease

    Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
    Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
    Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
    Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
    Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities

    Belongs to the nucleoporin GLFG family.
    Contains 1 peptidase S59 domain.
  • Domain

    Contains G-L-F-G repeats.
  • Post-translational
    modifications

    Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
  • Information by UniProt
  • Database links

    see all
  • Alternative names

    • 96 kDa nucleoporin antibody
    • 98 kDa nucleoporin antibody
    • ADAR2 antibody
    • ADIR2 antibody
    • GLFG-repeat containing nucleoporin antibody
    • Nuclear pore complex protein Nup96 antibody
    • Nuclear pore complex protein Nup98 Nup96 antibody
    • Nucleoporin 98kD antibody
    • nucleoporin 98kDa antibody
    • Nucleoporin Nup96 antibody
    • Nucleoporin Nup98 antibody
    • NUP196 antibody
    • NUP96 antibody
    • Nup98 antibody
    • Nup98-Nup96 antibody
    • NUP98_HUMAN antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells labeling NUP98 with ab179894 at 0.5 µg/ml in PBS (top panel). DAPI staining (middle panel). 4 μg/ml of Alexa488-labeled anti-mouse IgG was used as secondary antibody. The color image represents merged images of ab179894 (green) with DAPI (magenta).

  • Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894) at 0.4 µg/ml + HeLa cell extract

    Secondary
    HRP-labeled anti-mouse IgG at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Immunofluorescent analysis of Tetrahymena thermophila cells labeling NUP98 with ab179894 at 0.5 µg/ml (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta).4 μg/ml of Alexa488-labelled anti-mouse IgG was used as secondary antibody. Dotted lines represent the outlines of cells. The insert is a magnified image showing the position of the micronucleus.

  • All lanes : Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Lane 1 : Tetrahymena thermophila wild type cell extract
    Lane 2 : Tetrahymena thermophila cell extract ectopically-expressing GFP-MacNup98A (in addition to endogenous MacNup98A)

    Predicted band size: 112 kDa



    Open arrowheads represent the positions of MacNup98A. The 125-kDa band corresponds to GFP-MacNup98A. Diamonds and asterisks represent uncharacterized proteins.

  • Immunofluorescent analysis of S. pombe cells labeling NUP98 with ab179894 (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

  • All lanes : Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Lane 1 : S. pombe cell extract
    Lane 2 : NUP98-GFP-expressing S. pombe cell extract

    Predicted band size: 112 kDa

  • Immunofluorescent analysis of S. cerevisiae cells labeling NUP98 with ab179894 at 1/100 dilution (top panel). DAPI (middle panel). The color image represents a merged image of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

  • Supernatant of hybridoma culture medium of ab179894 at 1/10 dilution + S. cerevisiae cell extract

    Predicted band size: 112 kDa



    ab179894 crossreacts with multiple nucleoporins of S. cerevisiae.

    Asterisks represent uncharacterized proteins.

References

This product has been referenced in:

  • Iwamoto M  et al. Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans. Monoclon Antib Immunodiagn Immunother 32:81-90 (2013). Read more (PubMed: 23607342) »
See 1 Publication for this product

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