Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909)
Key features and details
- Mouse monoclonal [21A10] to NUP98
- Suitable for: WB, ICC/IF, Functional Studies
- Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
- Isotype: IgG1
Overview
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Product name
Anti-NUP98 antibody [21A10] - BSA and Azide free
See all NUP98 primary antibodies -
Description
Mouse monoclonal [21A10] to NUP98 -
Host species
Mouse -
Specificity
ab179909 crossreacts with multiple nucleoproteins of S. cerevisiae, e.g. Nup116, Nup100, Nup145N, Nup57 and Nup9. -
Tested applications
Suitable for: WB, ICC/IF, Functional Studiesmore details -
Species reactivity
Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
Sequence:MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ
Database link: D3KYQ3
Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664.
Sequence: SNPTQGGGLFGAANPGLGG
Database link: D3KYQ3 -
Epitope
GLF -
Positive control
- Tetrahymena thermophila, S. pombe and S. cerevisiae cells and cell extracts. HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Filter-sterilized. -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
21A10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.4 - 2 µg/ml. Predicted molecular weight: 112 kDa.
NOT suitable for Human samples. |
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ICC/IF |
Use a concentration of 0.5 - 10 µg/ml.
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Functional Studies |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 0.4 - 2 µg/ml. Predicted molecular weight: 112 kDa. NOT suitable for Human samples. |
ICC/IF
Use a concentration of 0.5 - 10 µg/ml. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
Involvement in disease
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
Sequence similarities
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
Domain
Contains G-L-F-G repeats. -
Post-translational
modificationsIsoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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Database links
- Entrez Gene: 4928 Human
- Entrez Gene: 852788 Saccharomyces cerevisiae
- Entrez Gene: 2541547 Schizosaccharomyces pombe
- Entrez Gene: 7832586 Tetrahymena
- Omim: 601021 Human
- SwissProt: P52948 Human
- SwissProt: P49687 Saccharomyces cerevisiae
- SwissProt: Q9UTK4 Schizosaccharomyces pombe
see all -
Alternative names
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
Images
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Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 0.4 µg/ml + HeLa cell extract
Secondary
HRP-labeled anti-mouse IgG at 0.4 µg/ml
Developed using the ECL technique.
Predicted band size: 112 kDaImage shows unsuitability of this antibody for Human samples.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.
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Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.
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All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml
All lanes : Tetrahymena thermophila cell extract
Developed using the ECL technique.
Predicted band size: 112 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?Open arrowheads correspond to NUP98. Asterisk represents uncharacterized protein. For lane 2 exposure time was about 10 times longer than for lane 1.
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Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.
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All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml
Lane 1 : Cell extract from S. pombe wild type
Lane 2 : Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence protein
Developed using the ECL technique.
Predicted band size: 112 kDa -
Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.
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Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml + S. cerevisiae cell extract
Developed using the ECL technique.
Predicted band size: 112 kDa -
Summary of the suitability of ab179909 for immunological applications. IF: indirect immunofluorescence staining; WB: Western blotting analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab179909 has been referenced in 1 publication.
- Iwamoto M et al. Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans. Monoclon Antib Immunodiagn Immunother 32:81-90 (2013). WB, ICC/IF ; Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe . PubMed: 23607342