Overview

  • Product name

    Anti-NUP98 antibody [21A10] - BSA and Azide free
    See all NUP98 primary antibodies
  • Description

    Mouse monoclonal [21A10] to NUP98 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    ab179909 crossreacts with multiple nucleoproteins of S. cerevisiae, e.g. Nup116, Nup100, Nup145N, Nup57 and Nup9.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
    Sequence:

    MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ


    Database link: D3KYQ3

    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664.
    Sequence: SNPTQGGGLFGAANPGLGG
    Database link: D3KYQ3

  • Epitope

    GLF
  • Positive control

    • Tetrahymena thermophila, S. pombe and S. cerevisiae cells and cell extracts. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab179909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 - 2 µg/ml. Predicted molecular weight: 112 kDa.

NOT suitable for Human samples.

ICC/IF Use a concentration of 0.5 - 10 µg/ml.

Target

  • Function

    Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
  • Involvement in disease

    Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
    Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
    Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
    Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
    Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities

    Belongs to the nucleoporin GLFG family.
    Contains 1 peptidase S59 domain.
  • Domain

    Contains G-L-F-G repeats.
  • Post-translational
    modifications

    Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
  • Information by UniProt
  • Database links

    see all
  • Alternative names

    • 96 kDa nucleoporin antibody
    • 98 kDa nucleoporin antibody
    • ADAR2 antibody
    • ADIR2 antibody
    • GLFG-repeat containing nucleoporin antibody
    • Nuclear pore complex protein Nup96 antibody
    • Nuclear pore complex protein Nup98 Nup96 antibody
    • Nucleoporin 98kD antibody
    • nucleoporin 98kDa antibody
    • Nucleoporin Nup96 antibody
    • Nucleoporin Nup98 antibody
    • NUP196 antibody
    • NUP96 antibody
    • Nup98 antibody
    • Nup98-Nup96 antibody
    • NUP98_HUMAN antibody
    see all

Images

  • Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 0.4 µg/ml + HeLa cell extract

    Secondary
    HRP-labeled anti-mouse IgG at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 112 kDa



    Image shows unsuitability of this antibody for Human samples.

  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged colored images.

  • Immunofluorescence analysis of methanol-fixed Tetrahymena thermophila cells, labeling NUP98 using ab179909 at 0.5 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green) at 4 µg/ml. DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells. The open arrow indicates the micronucleus. Insets are magnified images showing the position of the micronucleus.

  • All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml

    All lanes : Tetrahymena thermophila cell extract

    Developed using the ECL technique.

    Predicted band size: 112 kDa
    Observed band size: 98 kDa
    why is the actual band size different from the predicted?



    Open arrowheads correspond to NUP98. Asterisk represents uncharacterized protein. For lane 2 exposure time was about 10 times longer than for lane 1.

  • Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. pombe cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

  • All lanes : Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml

    Lane 1 : Cell extract from S. pombe wild type
    Lane 2 : Cell extract from S. pombe expressing endogenously NUP98 fused to a fluorescence protein

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Immunofluorescence analysis of formaldehyde-fixed, zymolyase-treated, S. cerevisiae cells, labeling NUP98 using ab179909 at 10 µg/ml, followed by Alexa Fluor 488-conjugated anti-mouse lgG (green). DAPI was used to stain DNA (magenta). Upper and middle panels correspond to black-and-white images while the bottom panel represents merged images. Dotted lines represent the outlines of cells.

  • Anti-NUP98 antibody [21A10] - BSA and Azide free (ab179909) at 2 µg/ml + S. cerevisiae cell extract

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Summary of the suitability of ab179909 for immunological applications. IF: indirect immunofluorescence staining; WB: Western blotting analysis.

References

This product has been referenced in:

  • Iwamoto M  et al. Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans. Monoclon Antib Immunodiagn Immunother 32:81-90 (2013). WB, ICC/IF ; Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe . Read more (PubMed: 23607342) »
See 1 Publication for this product

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