Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)
Key features and details
- Rat monoclonal [2H10] to NUP98 - Nuclear Pore Marker
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2c
Overview
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Product name
Anti-NUP98 antibody [2H10] - Nuclear Pore Marker
See all NUP98 primary antibodies -
Description
Rat monoclonal [2H10] to NUP98 - Nuclear Pore Marker -
Host species
Rat -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human NUP98 aa 1-466.
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Positive control
- WB: HeLa nuclear lysate. Jurkat, HeLa, COS-7, NIH/3T3 and SH-SY5Y cell lysate. ICC/IF: HeLa and NIH/3T3 cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2H10 -
Myeloma
Sp2 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab50610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 98 kDa. | |
| ICC/IF | Use at an assay dependent concentration. Customers have reported that Paraformaldehyde/Triton x-100 fixation provides better results, with sharp, regularly punctuate perinuclear signals. In MetOH fixed cells, the signal intensity can be somehwat lower and fuzzier and that single nucleoporin dots can be harder to distinguish around nuclear chromatin. Please see images below. |
Target
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Function
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
Involvement in disease
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
Sequence similarities
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
Domain
Contains G-L-F-G repeats. -
Post-translational
modificationsIsoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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Database links
- Entrez Gene: 4928 Human
- Entrez Gene: 269966 Mouse
- Entrez Gene: 81738 Rat
- Omim: 601021 Human
- SwissProt: P52948 Human
- SwissProt: Q6PFD9 Mouse
- SwissProt: P49793 Rat
- Unigene: 524750 Human
see all -
Alternative names
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)](https://www.abcam.com/ps/products/50/ab50610/Images/ab50610-336877-anti-nup98-antibody-2h10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)Di Nunzio et al PLoS One. 2012;7(9):e46037. doi: 10.1371/journal.pone.0046037. Epub 2012 Sep 25. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Lentiviral vector-encoded shRNAs achieve efficient knock-down of human nucleoporins and have negligible cytotoxic or cytostatic effects.
HeLa cells (4×106) were transduced with lentiviral vectors (MOI 50) encoding shRNAs specific for the indicated nucleoporins and used at 2 days p.t for Nup153 shRNA and 5 days p.t for all others.
(Panel B) Subcellular localisation of nuclear pore components upon nucleoporin knock-down was tested by confocal fluorescence microscopy of LV- (Control) and LV-shRNA transduced cells using specific anti-Nup antibodies. Images were acquired on the same day with the same conditions and are representative of two independent experiments.
This image was generated using the ascites version of the product.
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![Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)](https://www.abcam.com/ps/products/50/ab50610/Images/ab50610-283141-anti-nup98-antibody-2h10-western-blot.jpg)
Western blot - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)All lanes : Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 7 : P19 cell lysate
Lane 8 : NRK cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Developed using the ECL technique.This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)](https://www.abcam.com/ps/products/50/ab50610/Images/ab50610-62297-anti-nup98-antibody-2h10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italyab50610 (1/100) staining NUP98 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (green).
Cells were fixed with paraformaldehyde/Triton X-100 [10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature] or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)](https://www.abcam.com/ps/products/50/ab50610/Images/ab50610-62296-anti-nup98-antibody-2h10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)Image and protocol courtesy of Rosamaria Mangiacasale, Marilena Ciciarello and Patrizia Lavia, Univ Rome La Sapienza, Italyab50610 (1/100) staining NUP98 in NIH/3T3 (Mouse embryo fibroblast cell line) cells (green).
Cells were fixed with paraformaldehyde/Triton X-100 (10 min in PTEMF buffer (20mM PIPES, 1mM MgCl2, 10mM EGTA, 4% PFA) /0.2% Triton X-100 at room temperature) or methanol (6 min in methanol -20 °C , followed by 3 washes in 1x PBS) and counterstained with DAPI in order to highlight the nucleus (blue).
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)](https://www.abcam.com/ps/products/50/ab50610/Images/ab50610-283142-anti-nup98-antibody-2h10-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [2H10] - Nuclear Pore Marker (ab50610)This image is courtesy of an anonymous Abreview.Paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells stained for NUP98 (green) using ab50610 at 1/200 dilution in ICC/IF, followed by Donkey Anti-Rat Alexa Fluor® 488.
This image was generated using the ascites version of the product.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (14)
ab50610 has been referenced in 14 publications.
- Paonessa F et al. Microtubules Deform the Nuclear Membrane and Disrupt Nucleocytoplasmic Transport in Tau-Mediated Frontotemporal Dementia. Cell Rep 26:582-593.e5 (2019). PubMed: 30650353
- Zhang K et al. Stress Granule Assembly Disrupts Nucleocytoplasmic Transport. Cell 173:958-971.e17 (2018). PubMed: 29628143
- Balmus G et al. Targeting of NAT10 enhances healthspan in a mouse model of human accelerated aging syndrome. Nat Commun 9:1700 (2018). PubMed: 29703891
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Lång A et al. Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites. Nucleus 8:404-420 (2017). PubMed: 28402725
