Product nameAnti-NUP98 antibody - C-terminal
See all NUP98 primary antibodies
DescriptionRabbit polyclonal to NUP98 - C-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human NUP98 (C terminal). The exact sequence is proprietary.
Database link: P52948
- IP: HeLa whole cell extract. WB: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 198 kDa.|
|IP||1/100 - 1/500.|
FunctionNup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
Involvement in diseaseNote=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
Sequence similaritiesBelongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain.
DomainContains G-L-F-G repeats.
modificationsIsoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
- Information by UniProt
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
Anti-NUP98 antibody - C-terminal (ab228667) at 1/1000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 198 kDa
NUP98 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab228667. Western blot was performed from the immunoprecipitate using ab228667.
Lane 1: HeLa whole cell extract (Input).
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab228667 IP in HeLa whole cell extract.
ab228667 has not yet been referenced specifically in any publications.