Overview

  • Product name

    Anti-NUP98 antibody - C-terminal
    See all NUP98 primary antibodies
  • Description

    Rabbit polyclonal to NUP98 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human NUP98 (C terminal). The exact sequence is proprietary.
    Database link: P52948

  • Positive control

    • IP: HeLa whole cell extract. WB: Jurkat whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 198 kDa.
IP 1/100 - 1/500.

Target

  • Function

    Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
  • Involvement in disease

    Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
    Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
    Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
    Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
    Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
  • Sequence similarities

    Belongs to the nucleoporin GLFG family.
    Contains 1 peptidase S59 domain.
  • Domain

    Contains G-L-F-G repeats.
  • Post-translational
    modifications

    Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
  • Information by UniProt
  • Database links

  • Alternative names

    • 96 kDa nucleoporin antibody
    • 98 kDa nucleoporin antibody
    • ADAR2 antibody
    • ADIR2 antibody
    • GLFG-repeat containing nucleoporin antibody
    • Nuclear pore complex protein Nup96 antibody
    • Nuclear pore complex protein Nup98 Nup96 antibody
    • Nucleoporin 98kD antibody
    • nucleoporin 98kDa antibody
    • Nucleoporin Nup96 antibody
    • Nucleoporin Nup98 antibody
    • NUP196 antibody
    • NUP96 antibody
    • Nup98 antibody
    • Nup98-Nup96 antibody
    • NUP98_HUMAN antibody
    see all

Images

  • Anti-NUP98 antibody - C-terminal (ab228667) at 1/1000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 198 kDa



    7.5% SDS-PAGE

  • NUP98 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab228667. Western blot was performed from the immunoprecipitate using ab228667.

    Lane 1: HeLa whole cell extract (Input).

    Lane 2: Control IgG IP in HeLa whole cell extract.

    Lane 3: ab228667 IP in HeLa whole cell extract.

     

References

ab228667 has not yet been referenced specifically in any publications.

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