Overview

  • Product name

    Anti-NUP98 antibody [EPR22818-128]
    See all NUP98 primary antibodies
  • Description

    Rabbit monoclonal [EPR22818-128] to NUP98
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human NUP98 aa 700-900. The exact sequence is proprietary.
    Database link: P52948

  • Positive control

    • WB: HeLa, Jurkat, MCF7, HepG2, NIH/3T3, A20, PC-12, RAW264.7 whole cell lysate. Mouse testis and kidney tissue lysate. Human testis tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 198 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
    • Involvement in disease

      Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
      Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
      Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
      Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
      Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
    • Sequence similarities

      Belongs to the nucleoporin GLFG family.
      Contains 1 peptidase S59 domain.
    • Domain

      Contains G-L-F-G repeats.
    • Post-translational
      modifications

      Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
    • Cellular localization

      Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
    • Information by UniProt
    • Database links

    • Alternative names

      • 96 kDa nucleoporin antibody
      • 98 kDa nucleoporin antibody
      • ADAR2 antibody
      • ADIR2 antibody
      • GLFG-repeat containing nucleoporin antibody
      • Nuclear pore complex protein Nup96 antibody
      • Nuclear pore complex protein Nup98 Nup96 antibody
      • Nucleoporin 98kD antibody
      • nucleoporin 98kDa antibody
      • Nucleoporin Nup96 antibody
      • Nucleoporin Nup98 antibody
      • NUP196 antibody
      • NUP96 antibody
      • Nup98 antibody
      • Nup98-Nup96 antibody
      • NUP98_HUMAN antibody
      see all

    Images

    • All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution

      Lane 1 : Mouse testis tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Human testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lane 3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 198 kDa
      Observed band size: 98 kDa
      why is the actual band size different from the predicted?



      Exposure time: Lanes 1-2: 15 seconds Lane 3: 5.5 seconds.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 6 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
      Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 8 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 198 kDa
      Observed band size: 98 kDa why is the actual band size different from the predicted?


      Exposure time: 48 seconds


      Recommend using fresh lysates to avoid target protein being degraded.

      Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

    References

    ab234434 has not yet been referenced specifically in any publications.

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