Recombinant Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)
![Western blot - Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)](https://www.abcam.com/ps/products/256/ab256552/Images/ab256552-353154-AntiNUP98-antibody-EPR22818128--BSA-and-Azide-free-western-blot-testis-kidney-mouse-human.jpg "Western blot - Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22818-128] to NUP98 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free
See all NUP98 primary antibodies -
Description
Rabbit monoclonal [EPR22818-128] to NUP98 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, MCF7, HepG2, NIH/3T3, A20, PC-12, RAW264.7 whole cell lysate. Mouse testis and kidney tissue lysate. Human testis tissue lysate.
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General notes
ab256552 is the carrier-free version of ab234434.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22818-128 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
|
WB
Use at an assay dependent concentration. |
Target
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Function
Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport. -
Involvement in disease
Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4. -
Sequence similarities
Belongs to the nucleoporin GLFG family.
Contains 1 peptidase S59 domain. -
Domain
Contains G-L-F-G repeats. -
Post-translational
modificationsIsoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96. -
Cellular localization
Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket. - Information by UniProt
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Database links
- Entrez Gene: 4928 Human
- Entrez Gene: 269966 Mouse
- Entrez Gene: 81738 Rat
- Omim: 601021 Human
- SwissProt: P52948 Human
- SwissProt: Q6PFD9 Mouse
- SwissProt: P49793 Rat
- Unigene: 524750 Human
see all -
Alternative names
- 96 kDa nucleoporin antibody
- 98 kDa nucleoporin antibody
- ADAR2 antibody
see all
Images
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Western blot - Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)All lanes : Anti-NUP98 antibody [EPR22818-128] (ab234434) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Observed band size: 98 kDa why is the actual band size different from the predicted?Exposure time: Lanes 1-2: 15 seconds Lane 3: 5.5 seconds.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234434).
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![Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)](https://www.abcam.com/ps/products/256/ab256552/Images/ab256552-2-benefits-of-recombinant-antibodies.png)
Anti-NUP98 antibody [EPR22818-128] - BSA and Azide free (ab256552)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab256552 has not yet been referenced specifically in any publications.
