Recombinant Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980)


  • Product name

    Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker
    See all NUP98 primary antibodies
  • Description

    Rabbit monoclonal [EPR6678] to NUP98 - Nuclear Pore Marker
  • Host species

  • Specificity

    ab124980 recognises Nuclear pore complex protein Nup96 of Nuclear pore complex protein Nup98-Nup96.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NUP98 aa 1600-1700. The exact sequence is proprietary. The immunogen sequence is within Nuclear pore complex protein Nup96 of the Nuclear pore complex protein Nup98-Nup96.
    Database link: P52948

  • Positive control

    • HeLa, SH-SY5Y, and Caco-2 cell lysates and Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 188 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Nup98 and Nup96 play a role in the bidirectional transport across the nucleoporin complex (NPC). The repeat domain in Nup98 has a direct role in the transport.
    • Involvement in disease

      Note=A chromosomal aberration involving NUP98 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with HOXA9. Translocation t(11;17)(p15;p13) with PHF23.
      Note=A chromosomal aberration involving NUP98 is found in childhood acute myeloid leukemia. Translocation t(5;11)(q35;p15.5) with NSD1. Translocation t(8;11)(p11.2;p15) with WHSC1L1.
      Note=A chromosomal aberration involving NUP98 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with TOP1.
      Note=A chromosomal aberration involving NUP98 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(3;11)(q12.2;p15.4) with LNP1.
      Note=A chromosomal aberration involving NUP98 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with PSIP1/LEDGF. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
    • Sequence similarities

      Belongs to the nucleoporin GLFG family.
      Contains 1 peptidase S59 domain.
    • Domain

      Contains G-L-F-G repeats.
    • Post-translational

      Isoform 1 to isoform 4 are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively. Cleaved Nup98 is necessary for the targeting of Nup98 to the nuclear pore and the interaction with Nup96.
    • Cellular localization

      Nucleus > nuclear pore complex. Nucleus membrane. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex, at or near the nucleoplasmic basket.
    • Information by UniProt
    • Database links

    • Alternative names

      • 96 kDa nucleoporin antibody
      • 98 kDa nucleoporin antibody
      • ADAR2 antibody
      • ADIR2 antibody
      • GLFG-repeat containing nucleoporin antibody
      • Nuclear pore complex protein Nup96 antibody
      • Nuclear pore complex protein Nup98 Nup96 antibody
      • Nucleoporin 98kD antibody
      • nucleoporin 98kDa antibody
      • Nucleoporin Nup96 antibody
      • Nucleoporin Nup98 antibody
      • NUP196 antibody
      • NUP96 antibody
      • Nup98 antibody
      • Nup98-Nup96 antibody
      • NUP98_HUMAN antibody
      see all


    • All lanes : Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker (ab124980) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : SH-SY5Y cell lysates
      Lane 3 : Caco-2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 188 kDa

    • ab124980 staining NUP98 in SH-SY5Y, human neuroblastoma epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500). Ab150077, an Alexa Fluor® 594-conjugated Goat anti rabbit (1:1000) (ab150077) was used as the secondary antibody. Counterstained with ab195889, an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (1/100). Nuclear counterstained with DAPI.

    • ab124980, at 1/50 to 1/100, staining NUP98 in Human colon tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].


    This product has been referenced in:

    • Light WH  et al. A conserved role for human Nup98 in altering chromatin structure and promoting epigenetic transcriptional memory. PLoS Biol 11:e1001524 (2013). Read more (PubMed: 23555195) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections)
    Mouse Tissue sections (postnatal 60days brain)
    Yes - 0.4%triton 30min
    postnatal 60days brain
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

    Dr. liang zhang

    Verified customer

    Submitted Feb 03 2017

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