Immunohistochemistry (Frozen sections) abreview for Anti-NUP98 antibody [EPR6678] - Nuclear Pore Marker

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (postnatal 60days brain)
Permeabilization
Yes - 0.4%triton 30min
Specification
postnatal 60days brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1%triton/1%bsa/pbs
Dilution
1/200

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: goat anti-rabbit cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ­

Additional data

Additional Notes
4%pfa 8h. heat-antigen retrieval for 10min. Although 1/200 diluted, it can be diluted further.

Dr. liang zhang

Verified customer

Submitted Feb 03 2017

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