Key features and details
- Rabbit polyclonal to NUPL1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NUPL1 antibody
DescriptionRabbit polyclonal to NUPL1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human NUPL1 aa 211-480.
Database link: Q9BVL2
- WB: HepG2, HeLa and L02 whole cell lysates; Mouse thymus tissue lysate. IHC-P: Human small intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 61 kDa.|
|IHC-P||1/20 - 1/200.|
RelevanceNUPL1 is a member of the nucleoporin family. It is a component of the nuclear pore complex, a complex required for trafficking across the nuclear membrane.
Cellular localizationNucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter.
- KIAA0410 antibody
- Nucleoporin like 1 antibody
- Nucleoporin p58/p45 antibody
Paraffin-embedded human small intestine tissue stained for NUPL1 using ab235371 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-NUPL1 antibody (ab235371) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : L02 (human immortal liver cell line) whole cell lysate
Lane 4 : Mouse thymus tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 61 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235371 has not yet been referenced specifically in any publications.