Overview

  • Product name
    Anti-NUR77 antibody - ChIP Grade
    See all NUR77 primary antibodies
  • Description
    Rabbit polyclonal to NUR77 - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ChIP, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human NUR77 aa 251-266.
    Sequence:

    TSTKARSGAPGGSEGR


    Database link: P22736

  • Positive control
    • WB: Recombinant Human NUR77 protein (ab114314), NIH/3T3 cell lysate. ICC/IF: Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab13851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/2000. PubMed: 21726557
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa.

Target

  • Function
    Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
  • Tissue specificity
    Fetal muscle and adult liver, brain and thyroid.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Early response protein NAK1 antibody
    • GFRP 1 antibody
    • GFRP antibody
    • GFRP1 antibody
    • Growth factor inducible nuclear protein N10 antibody
    • Growth Factor Inducible Nuclear Protein NP10 antibody
    • Growth Factor Response Protein 1 antibody
    • Hbr1 antibody
    • HMR antibody
    • Hormone Receptor antibody
    • MGC9485 antibody
    • N10 antibody
    • N10 nuclear protein antibody
    • NAK 1 antibody
    • NAK1 antibody
    • Nerve growth factor IB nuclear receptor variant 1 antibody
    • NGFIB antibody
    • NP 10 antibody
    • NP10 antibody
    • NR4A1 antibody
    • NR4A1_HUMAN antibody
    • Nuclear hormone receptor NUR/77 antibody
    • Nuclear Hormone Receptor TR3 antibody
    • Nuclear receptor subfamily 4 group A member 1 antibody
    • NUR77 antibody
    • NUR77, mouse, homolog of antibody
    • Orphan nuclear receptor HMR antibody
    • Orphan nuclear receptor NR4A1 antibody
    • Orphan nuclear receptor TR3 antibody
    • Orphan receptor tr3 antibody
    • Receptor NGFIB antibody
    • ST 59 antibody
    • ST-59 antibody
    • ST59 antibody
    • Steroid receptor TR3 antibody
    • Testicular receptor 3 antibody
    • TR 3 antibody
    • TR3 antibody
    • TR3 orphan receptor antibody
    see all

Images

  • Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained for NUR77 using ab13851 at a dilution of 1/50 in ICC/IF. Secondary used was a Dylight®488 conjugate (green). Alpha tubulin and nuclei were counterstained against Dylight®568 (Red) and DAPI (Blue), respectively.

  • Anti-NUR77 antibody - ChIP Grade (ab13851) at 1/500 dilution + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 15 µg

References

This product has been referenced in:
  • Parvin R  et al. Inhibitory Effects of a Novel PPAR-? Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells. PPAR Res 2018:5346272 (2018). Read more (PubMed: 29849538) »
  • Takeda Y  et al. Epigenetic Regulation of Aldosterone Synthase Gene by Sodium and Angiotensin II. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29739797) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (DO11.10 thymocyte hybridoma)
Loading amount
25 µg
Specification
DO11.10 thymocyte hybridoma
Treatment
PMA/Ionomycin
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Jonathan Rud

Verified customer

Submitted Dec 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T cells over expressing Nur77)
Total protein in input
25 µg
Specification
HEK 293T cells over expressing Nur77
Treatment
transfected with Nur77 expression construct
Immuno-precipitation step
Protein A/G

Dr. Jonathan Rud

Verified customer

Submitted Dec 30 2009

Answer

Thank you for filling out our technical help questionnaire. The non-specific band you are seeing may very well be NUR77. The following reference describes the appearance of the protein in Western blots at sizes ranging from 63 - 88 kD, due to post-translational modifications: Fahrner TJ, et al. The NGFI-B protein, an inducible member of the thyroid/steroid receptor family, is rapidly modified posttranslationally. Mol Cell Biol. 1990 Dec;10(12):6454-9. PMID: 2247065 The article can be found at this link: http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=2247065 The note on the datasheet for ab41913 refers to the predicted size, based on the 64 kD molecular weight of the precursor, but the size of the band(s) on the blot will likely be heavier than this.

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Answer

Thank you for your enquiry. Ab13851 - Rabbit polyclonal to NUR77 - was generated using the following peptide: TSTKARSGAPGGSEGR, corresponding to amino acids 251-266 of Human NUR77. This antibody cross-reacts with human and mouse and is predicted (it has not yet been tested) to react with Rat (87% identity with immunogen) due to sequence homology. This antibody works in Western blot (there is an image on the online datasheet) and has not yet been tested in other applications. We're currently not aware of any publications which have featured the use of ab13851. We also have ab1406 - Rabbit polyclonal to NUR77. Please note that ab1406 is a fast-track antibody and has not yet been fully characterized. More information about this can be found on the online product datasheet. Please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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