Recombinant Anti-NUR77 antibody [EPR3209] (ab109180)
- Datasheet
- References (12)
- Protocols
Overview
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Product name
Anti-NUR77 antibody [EPR3209]
See all NUR77 primary antibodies -
Description
Rabbit monoclonal [EPR3209] to NUR77 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human NUR77 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- SH-SY5Y cell lysate and HepG2 cells and lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.11% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3209 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. -
Tissue specificity
Fetal muscle and adult liver, brain and thyroid. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3164 Human
- Entrez Gene: 15370 Mouse
- Entrez Gene: 79240 Rat
- Omim: 139139 Human
- SwissProt: P22736 Human
- SwissProt: P12813 Mouse
- SwissProt: P22829 Rat
- Unigene: 524430 Human
see all -
Alternative names
- Early response protein NAK1 antibody
- GFRP 1 antibody
- GFRP antibody
see all
Images
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![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-261834-anti-nur77-antibody-epr3209-western-blot.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-121749-anti-nur77-antibody-epr3209-western-blot.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)All lanes : Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa -
![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-261835-anti-nur77-antibody-epr3209-western-blot.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Mouse pituitary at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-261836-anti-nur77-antibody-epr3209-western-blot.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Rat brain at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST
Protocols
References
This product has been referenced in:
- Mey JT et al. Skeletal muscle Nur77 and NOR1 insulin responsiveness is blunted in obesity and type 2 diabetes but improved after exercise training. Physiol Rep 7:e14042 (2019). Read more (PubMed: 30912283) »
- Zhang Z et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 30297870) »
