Anti-NUR77 antibody [EPR3209] (ab109180)
- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-NUR77 antibody [EPR3209]
See all NUR77 primary antibodies -
DescriptionRabbit monoclonal [EPR3209] to NUR77
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human NUR77 aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- SH-SY5Y cell lysate and HepG2 cells and lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.11% BSA, PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR3209
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
Target
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FunctionOrphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.
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Tissue specificityFetal muscle and adult liver, brain and thyroid.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 3164 Human
- Entrez Gene: 15370 Mouse
- Entrez Gene: 79240 Rat
- Omim: 139139 Human
- SwissProt: P22736 Human
- SwissProt: P12813 Mouse
- SwissProt: P22829 Rat
- Unigene: 524430 Human
see all -
Alternative names
- Early response protein NAK1 antibody
- GFRP 1 antibody
- GFRP antibody
see all
Images
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![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-3-ab109180WB1.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/NUR77-Primary-antibodies-ab109180-3.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)All lanes : Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 64 kDa
Observed band size: 64 kDa -
![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-4-ab109180WB2.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Mouse pituitary at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
![Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)](https://www.abcam.com/ps/products/109/ab109180/Images/ab109180-5-ab109180WB3.jpg)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Rat brain at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/Diluting buffer 5% NFDM/TBST
Protocols
References
This product has been referenced in:
- Shi X et al. The involvement and possible mechanism of NR4A1 in chondrocyte apoptosis during osteoarthritis. Am J Transl Res 9:746-754 (2017). Read more (PubMed: 28337303) »
- Liu NA et al. Cyclin E - mediated human proopiomelanocortin regulation as a therapeutic target for Cushing disease. J Clin Endocrinol Metab N/A:jc20151606 (2015). WB ; Human . Read more (PubMed: 25942479) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"