RecombinantRabMAb

Recombinant Anti-NUR77 antibody [EPR3209] (ab109180)

Submit a review Q&A (1)References (14)
Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
  • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
  • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
  • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
  • Anti-NUR77 antibody [EPR3209] (ab109180)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3209] to NUR77
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NUR77 antibody [EPR3209]
    See all NUR77 primary antibodies

  • Description

    Rabbit monoclonal [EPR3209] to NUR77

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y cell lysate and HepG2 cells and lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2.

    • Tissue specificity

      Fetal muscle and adult liver, brain and thyroid.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR4 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Early response protein NAK1 antibody
      • GFRP 1 antibody
      • GFRP antibody
      • GFRP1 antibody
      • Growth factor inducible nuclear protein N10 antibody
      • Growth Factor Inducible Nuclear Protein NP10 antibody
      • Growth Factor Response Protein 1 antibody
      • Hbr1 antibody
      • HMR antibody
      • Hormone Receptor antibody
      • MGC9485 antibody
      • N10 antibody
      • N10 nuclear protein antibody
      • NAK 1 antibody
      • NAK1 antibody
      • Nerve growth factor IB nuclear receptor variant 1 antibody
      • NGFIB antibody
      • NP 10 antibody
      • NP10 antibody
      • NR4A1 antibody
      • NR4A1_HUMAN antibody
      • Nuclear hormone receptor NUR/77 antibody
      • Nuclear Hormone Receptor TR3 antibody
      • Nuclear receptor subfamily 4 group A member 1 antibody
      • NUR77 antibody
      • NUR77, mouse, homolog of antibody
      • Orphan nuclear receptor HMR antibody
      • Orphan nuclear receptor NR4A1 antibody
      • Orphan nuclear receptor TR3 antibody
      • Orphan receptor tr3 antibody
      • Receptor NGFIB antibody
      • ST 59 antibody
      • ST-59 antibody
      • ST59 antibody
      • Steroid receptor TR3 antibody
      • Testicular receptor 3 antibody
      • TR 3 antibody
      • TR3 antibody
      • TR3 orphan receptor antibody
      see all

    Images

    • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + HepG2 whole cell lysate at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      All lanes : Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 64 kDa
      Observed band size: 64 kDa

    • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Mouse pituitary at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Western blot - Anti-NUR77 antibody [EPR3209] (ab109180)
      Anti-NUR77 antibody [EPR3209] (ab109180) at 1/1000 dilution + Rat brain at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking/Diluting buffer 5% NFDM/TBST
    • Anti-NUR77 antibody [EPR3209] (ab109180)
      Anti-NUR77 antibody [EPR3209] (ab109180)

    References (14)

    Publishing research using ab109180? Please let us know so that we can cite the reference in this datasheet.

    ab109180 has been referenced in 14 publications.

    • Mey JT  et al. Skeletal muscle Nur77 and NOR1 insulin responsiveness is blunted in obesity and type 2 diabetes but improved after exercise training. Physiol Rep 7:e14042 (2019). PubMed: 30912283
    • Mirchi LF  et al. Transcriptomic analysis of left ventricle myocardium in an SHR congenic line with ameliorated cardiac fibrosis. Physiol Res 68:747-755 (2019). PubMed: 31424260
    • Mohankumar K  et al. Bis-Indole-Derived NR4A1 Ligands and Metformin Exhibit NR4A1-Dependent Glucose Metabolism and Uptake in C2C12 Cells. Endocrinology 159:1950-1963 (2018). PubMed: 29635345
    • Zhang Y  et al. Targeting nuclear receptor NR4A1-dependent adipocyte progenitor quiescence promotes metabolic adaptation to obesity. J Clin Invest 128:4898-4911 (2018). PubMed: 30277475
    • Zhou H  et al. NR4A1 aggravates the cardiac microvascular ischemia reperfusion injury through suppressing FUNDC1-mediated mitophagy and promoting Mff-required mitochondrial fission by CK2a. Basic Res Cardiol 113:23 (2018). PubMed: 29744594
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    To whom it may concern:

    I am troubleshooting using your monoclonal antibody ab109180 to identify Nur77 expression in human PBMCs. In mouse, Nur77 has been identified as a marker analogous to CD69, induced upon TCR signaling. I have tried using the antibody in a time course experiment in both flow cytometry and western blots. I was wondering if you can offer me a positive control or a peptide to block antibody binding so I can ask the question, does Nur77 serve as a read out of antigen stimulation in human T cells?

    Thank you for your time,

    Read More
    Answer

    Thank you for your email.
    Unfortunately we are not able to provide the immunogen peptide for this particular antibody, as the laboratory providing this antibody does not have it available. I am very sorry.
    In regards to positive control, I can recommend HepG2 cells, as they have provided good staining as shown on the datasheet. Otherwise, for mouse leukocytes, PMA and ionomycin treatment can induce Nur77 expression. I am not sure whether a similar stimulation could also induce expresssion on your cells?
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386419/?tool=pubmed
    I am sorry that I can not help more in this regards.
    If you would like to doublecheck the technical flow cytometry protocol together, I would be very pleased to do so. I have attached therefore a questionnaire with which you could easily provide me with the information I need for analysing the protocol.
    Please do let me know how you are proceeding and do not hesitate neither to contact me again, should you have any other question or concern.

    Read More

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