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    nurim-antibody-ab221972.pdf

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Tags & Cell Markers Subcellular Markers Nucleus Nuclear Envelope
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Anti-Nurim antibody (ab221972)

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Immunocytochemistry/ Immunofluorescence - Anti-Nurim antibody (ab221972)

    Key features and details

    • Rabbit polyclonal to Nurim
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-Nurim antibody
      See all Nurim primary antibodies
    • Description

      Rabbit polyclonal to Nurim
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Macaque monkey
    • Immunogen

      Recombinant fragment aa 169-196.
      Sequence:

      QVYYHVLGLGEPLALKSPRALRLFSHLR


      Database link: Q8IXM6
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • ICC/IF: RH-30 cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Nucleus
      • Nuclear Envelope

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab221972 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    • Sequence similarities

      Belongs to the nurim family.
    • Cellular localization

      Nucleus inner membrane.
    • Target information above from: UniProt accession Q8IXM6 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 471957 Chimpanzee
      • Entrez Gene: 511167 Cow
      • Entrez Gene: 11270 Human
      • Entrez Gene: 106582 Mouse
      • Entrez Gene: 100144454 Pig
      • Entrez Gene: 361791 Rat
      • SwissProt: Q1XHX8 Chimpanzee
      • SwissProt: Q32LM8 Cow
      • SwissProt: Q8IXM6 Human
      • SwissProt: Q8VC65 Mouse
      • SwissProt: Q767L9 Pig
      • SwissProt: Q6MG14 Rat
      • Unigene: 519993 Human
      • Unigene: 279713 Mouse
      • Unigene: 390992 Mouse
      • Unigene: 38521 Rat
      see all
    • Alternative names

      • NRM antibody
      • NRM_HUMAN antibody
      • NRM29 antibody
      • Nuclear envelope membrane protein antibody
      • Nuclear rim protein antibody
      • Nurim antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Nurim antibody (ab221972)
      Immunocytochemistry/ Immunofluorescence - Anti-Nurim antibody (ab221972)

      PFA fixed, Triton X-100 permeabilized RH-30 cells stained for Nurim (green) using ab221972 (4 µg/ml) in ICC/IF.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab221972? Please let us know so that we can cite the reference in this datasheet.

    ab221972 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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