Anti-Nurr1 antibody [447C2a] (ab54366)
Key features and details
- Mouse monoclonal [447C2a] to Nurr1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Nurr1 antibody [447C2a]
See all Nurr1 primary antibodies -
Description
Mouse monoclonal [447C2a] to Nurr1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Nurr1 recombinant fragment (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab54366 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
447C2a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 66 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 66 kDa). |
Target
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Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. -
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
see all
Images
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Anti-Nurr1 antibody [447C2a] (ab54366) + immunised recombinant protein
Predicted band size: 66 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab54366 has been referenced in 1 publication.
- Xu Y et al. Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes. Mol Med Rep 12:5487-93 (2015). PubMed: 26239742