Key features and details
- Mouse monoclonal [447C2a] to Nurr1
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab54366 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab54366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 37 kDa (predicted molecular weight: 66 kDa).|
|Dot blot||Use at an assay dependent dilution.|
FunctionTranscriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
Tissue specificityExpressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Developmental stageRapidly and only very transiently expressed after cell activation, during the G0-G1 transition of the cell cycle.
- Information by UniProt
- HZF 3 antibody
- HZF3 antibody
- Immediate-early response protein NOT antibody
Anti-Nurr1 antibody [447C2a] (ab54366) + immunised recombinant protein
Predicted band size: 66 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
ab54366 has been referenced in 1 publication.
- Xu Y et al. Decreased expression levels of Nurr1 are associated with chronic inflammation in patients with type 2 diabetes. Mol Med Rep 12:5487-93 (2015). PubMed: 26239742